Development of a DNA vaccine for chicken infectious anemia and its immunogenicity studies using high mobility group box 1 protein as a novel immunoadjuvant indicated induction of promising protective immune responses |
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| Institution: | 1. Immunology Section, Indian Veterinary Research Institute (IVRI), Izatnagar, Uttar Pradesh 243122, India;2. Avian Diseases Section Indian Veterinary Research Institute (IVRI) , Izatnagar, Uttar Pradesh 243122, India;3. Division of Veterinary Public Health, Indian Veterinary Research Institute (IVRI) , Izatnagar, Uttar Pradesh 243122, India;4. Department of Veterinary Microbiology, College of Veterinary Sciences, Pandit Deen Dayal Upadhaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, Uttar Pradesh, 281001, India;5. Indian Veterinary Research Institute (IVRI), Izatnagar, Uttar Pradesh 243122, India;1. Taylor University Counseling Center, 236 W. Reade Ave, Upland, IN 46989, USA;2. Department of Anthropology, Texas State University, 601 University Dr., San Marcos, TX 78666, USA;1. Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;2. State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;3. National Institute of Diagnostics and Vaccine Development in Infectious Disease, State Key Laboratory of Cellular Stress Biology, School of Life Science, Xiamen University, Xiamen 361005, China;4. Shanghai Institute of Biological Products, Shanghai 200052, China;5. Chinese Center for Disease Control and Prevention, Beijing 100052, China;1. FEPAGRO – Saúde Animal, Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul CEP 92990-000, Rio Grande do Sul, Brazil;2. Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, UFRGS. Av. Sarmento Leite 500, Sala 208, Porto Alegre CEP 90050-170, Rio Grande do Sul, Brazil;3. EMBRAPA Suínos e Aves, BR 153 Km 110, Concordia CEP: 89700-000, SC, Brazil;1. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, People’s Republic of China;2. Shapotou Center for Animal Disease Control and Prevention, NingXia, ZhongWei, Shapotou 755000, People’s Republic of China |
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| Abstract: | Chicken infectious anaemia (CIA) is an economically important and emerging poultry disease reported worldwide. Current CIA vaccines have limitations like, the inability of the virus to grow to high titres in embryos/cell cultures, possession of residual pathogenicity and a risk of reversion to virulence. In the present study, a DNA vaccine, encoding chicken infectious anaemia virus (CIAV) VP1 and VP2 genes, was developed and co-administered with truncated chicken high mobility group box 1 (HMGB1ΔC) protein in young chicks for the evaluation of vaccine immune response. CIAV VP1 and VP2 genes were cloned in pTARGET while HMGB1ΔC in PET32b vector. In vitro expression of these gene constructs was evaluated by Western blotting. Further, recombinant HMGB1ΔC was evaluated for its biological activity. The CIAV DNA vaccine administration in specific pathogen free chicks resulted in moderately protective ELISA antibody titres in the range of 4322.87 ± 359.72 to 8288.19 ± 136.38, increased CD8+ cells, and a higher titre was observed by co-administration of novel adjuvant (HMGB1ΔC) and booster immunizations. The use of vaccine with adjuvant showed achieving antibody titres nearly 8500, titre considered as highly protective, which indicates that co-immunization of HMGB1ΔC may have a strong adjuvant activity on CIAV DNA vaccine induced immune responses. The able potential of HMGB1 protein holding strong adjuvant activity could be exploited further with trials with vaccines for other important pathogens for achieving the required protective immune responses. |
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| Keywords: | Chicken infectious anaemia (CIA) DNA vaccine Adjuvant HMGB1 Immunogenicity |
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