Cross-protective efficacy of engineering serotype A foot-and-mouth disease virus vaccine against the two pandemic strains in swine |
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| Institution: | 1. Central Veterinary Institute of Wageningen UR (CVI), PO Box 65, 8200 AB Lelystad, The Netherlands;2. Faculty of Veterinary Medicine, University of Calgary,3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada;1. Iranian Veterinary Organization, Provincial Department of Surveillance and Disease Control, Urumiyah West Azerbaijan, Islamic Republic of Iran;2. Iranian Veterinary Organization, Central Department of Surveillance and Disease Control, Tehran, Islamic Republic of Iran;3. The European Commission for the Control of FMD, Rome, Italy;1. Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, POB 12, Rehovot 76100, Israel;2. Central Veterinary Institute, Part of Wageningen UR, Lelystad, The Netherlands;3. Kimron Veterinary Institute, Beit Dagan, Israel;1. Animal and Plant Quarantine Agency, 175 Anyang-ro, Manangu, Anyang city, Gyeonggido, 430-757, Republic of Korea;2. Gyeonggi Province Veterinary Service Center, Anseong-si, Gyeonggi-do, 456-823, Republic of Korea;3. Veterinary College, Chungnam National University, Yuseonggu, Daejeon, 305-764, Republic of Korea;1. The Pirbright Institute, Ash Road, Pirbright, Woking, GU24 0NF, UK;2. Met Office, FitzRoy Rd, Exeter, EX1 3PB, UK;3. Central Veterinary Institute (CVI), Wageningen UR, P.O. Box 65, 8200 AB Lelystad, The Netherlands, The Netherlands |
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| Abstract: | Foot-and-mouth disease (FMD) is a highly contagious vesicular disease that affects domestic and wild cloven-hoofed animals worldwide. Recently, a series of outbreaks of type A FMDV occurred in Southeast Asian countries, China, the Russia Federation, Mongolia, Kazakhstan and South Korea. The FMD virus (A/GDMM/CHA/2013) from China's Guangdong province (2013) is representative of those responsible for the latest epidemic, and has low amino acid identity (93.9%) in VP1 protein with the epidemic strain A/WH/CHA/09 from Wuhan, China in 2009. Both of isolates belong to the Sea-97 genotype of ASIA topotype. Therefore, the application of a new vaccine strain with cross-protective efficacy is of fundamental importance to control the spread of the two described pandemic strains. A chimeric strain rA/P1-FMDV constructed by our lab previously through replacing the P1 gene in the vaccine strain O/CHA/99 with that from the epidemic stain A/WH/CHA/09, has been demonstrated to exhibit good growth characteristics in culture, and the rA/P1-FMDV inactivated vaccine can provide protection against epidemic strain A/WH/CHA/09 in cattle. However, it is still unclear whether the vaccine produces efficient protection against the new pandemic strain (A/GDMM/CHA/2013). Here, vaccine matching and pig 50% protective dose (PD50) tests were performed to assess the vaccine potency. The vaccine matching test showed cross-reactivity of sera from full dose vaccine vaccinated pigs with A/WH/CHA/09 and A/GDMM/CHA/2013 isolates, with average r1 values of 0.94 ± 0.12 and 0.68 ± 0.06 (r1 ≥ 0.3), which indicates that the rA/P1-FMDV vaccine is likely to confer good cross-protection against the two isolates. When challenged with two pandemic isolates A/WH/CHA/09 and A/GDMM/CHA/2013 strain, the vaccine achieved 12.51 PD50 and 10.05 PD50 per dose (2.8 μg), respectively. The results indicated that the rA/P1-FMDV inactivated vaccine could protect pigs against both A/WH/CHA/09 and A/GDMM/CHA/2013 pandemic isolates. |
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| Keywords: | Foot-and-mouth disease virus ASIA topotype Engineering foot-and-mouth disease virus Protective efficacy |
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