Anti-skin cancer properties of phenolic-rich extract from the pericarp of mangosteen (Garcinia mangostana Linn.) |
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Authors: | Jing J. Wang Qing H. Shi Wei Zhang Barbara J.S. Sanderson |
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Affiliation: | 1. Level 4, Health Science Building, Department of Medical Biotechnology, Flinders Medical Sciences and Technology, School of Medicine, Faculty of Health Science, Flinders University, Registry Road, Bedford Park, Adelaide 5042, Australia;2. Level 4, Health Science Building, Flinders Centre for Marine Bioproducts Development (FCMBD), Flinders University, Registry Road, Bedford Park, Adelaide 5042, Australia;3. Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China |
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Abstract: | Skin cancers are often resistant to conventional chemotherapy. This study examined the anti-skin cancer properties of crude ethanol extract of mangosteen pericarp (MPEE) on human squamous cell carcinoma A-431 and melanoma SK-MEL-28 lines. Significant dose-dependent reduction in% viability was observed for these cell lines, with less effect on human normal skin fibroblast CCD-1064Sk and keratinocyte HaCaT cell lines. Cell distribution in G1 phase (93%) significantly increased after 10 μg/ml of MPEE versus untreated SK-MEL-28 cells (78%), which was associated with enhanced p21WAF1 mRNA levels. In A-431 cells, 10 μg/ml MPEE significantly increased the sub G1 peak (15%) with concomitant decrease in G1 phase over untreated cells (2%). In A-431 cells, 10 μg/ml MPEE induced an 18% increase in early apoptosis versus untreated cells (2%). This was via caspase activation (15-, 3- and 4-fold increased caspse-3/7, 8, and 9 activities), and disruption of mitochondrial pathways (6-fold decreased mitochondrial membrane potential versus untreated cells). Real-time PCR revealed increased Bax/Bcl-2 ratio and cytochrome c release, and decreased Akt1. Apoptosis was significantly increased after MPEE treatment of SK-MEL-28 cells. Hence, MPEE showed strong anti-skin cancer effect on these two skin cancer cell lines, with potential as an anti-skin cancer agent. |
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Keywords: | Skin cancer Mangosteen Cell cycle arrest Apoptosis induction Mitochondrial pathway Akt pathway |
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