Gentiana asclepiadea exerts antioxidant activity and enhances DNA repair of hydrogen peroxide- and silver nanoparticles-induced DNA damage |
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Authors: | Alexandra Hudecová Barbara Kusznierewicz Katarína Hašplová Anna Huk Zuzana Magdolenová Eva Miadoková Eliška Gálová Mária Dušinská |
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Institution: | 1. Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina, Bratislava 842 15, Slovakia;2. Centre for Ecology and Economics (CEE), Health Effects Group, Norwegian Institute for Air Research, P.O. Box 100, Kjeller 2027, Norway;3. Department of Food Chemistry, Technology and Biotechnology, Gdańsk University of Technology, 11/12 G. Narutowicza Str., Gdańsk 80-233, Poland |
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Abstract: | Exposure to high levels of different environmental pollutants is known to be associated with induction of DNA damage in humans. Thus DNA repair is of great importance in preventing mutations and contributes crucially to the prevention of cancer. In our study we have focused on quantitative analysis of Gentiana asclepiadea aqueous or methanolic extracts obtained from flower and haulm, their antioxidant potency in ABTS post-column derivatisation, and their potential ability to enhance DNA repair in human lymphocytes after hydrogen peroxide (H2O2) treatment (250 μM, 5 min). We also studied DNA repair in human kidney HEK 293 cells after exposure to 20 nm silver nanoparticles (AgNPs) (100 μg/ml, 30 min) in the presence and absence of the plant extract. We have found that mangiferin along with unidentified polar compounds are the most pronounced antioxidants in the studied extracts. Extract from haulm exhibited slightly stronger antioxidant properties compared to flower extracts. However, all four extracts showed significant ability to enhance DNA repair in both cell types after H2O2 and AgNP treatments. |
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Keywords: | DNA repair DNA damage Gentiana asclepiadea Hydrogen peroxide Silver nanoparticles Human cells |
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