A novel real-time PCR method for KIR genotyping |
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| Authors: | L. G. T. Alves,R. Rajalingam,& F. Canavez |
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| Affiliation: | Genetic Division, Genoa Biotechnology SA, São Paulo, SP, Brazil;
Department of Pathology and Laboratory Medicine, UCLA Immunogenetics Center, David Geffen School of Medicine at UCLA, University of California at Los Angeles, Los Angeles, CA, USA |
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| Abstract: | Genes encoding killer cell immunoglobulin-like receptors (KIRs) are variable among individuals. Sequence-specific primer-directed polymerase chain reaction (PCR) amplification (PCR-SSP) and sequence-specific oligonucleotide hybridization of the PCR-amplified products (PCR-SSO) are the methods currently used to characterize the diversity of KIR gene content. Both these methods include time-consuming post-PCR analyses. Here, we developed a real-time PCR method that identifies the presence or absence of 16 KIR genes during PCR and avoids post-PCR analyses. This method is specific, sensitive, shortens the turnaround time compared with the conventional PCR-SSP and PCR-SSO methods, and it can be easily adapted for automation. |
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| Keywords: | KIR genotyping noninvasive sampling real-time PCR SYBR green |
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