移植胚胎干细胞的衍生细胞修复小鼠脊髓损伤的行为学观察

目的：观察胚胎干细胞的衍生细胞移植对小鼠脊髓损伤神经功能恢复的影响.方法：将小鼠胚胎干细胞体外诱导分化为胚胎干细胞的衍生细胞后,制作小鼠脊髓半切损伤模型,在实验组注射胚胎干细胞的衍生细胞到脊髓半切小鼠的损伤脊髓周围.实验对照组按照同样方法注入等量磷酸盐缓冲液.移植后2、4、8周观察小鼠脊髓神经功能恢复情况（BBB评分）.结果：实验组较实验对照组死亡数下降（实验组2只,实验对照组7只）,并有明显的神经功能恢复（各时相点BBB评分及斜板试验临界角度两组间比较（P＜0.01）.结论：在脊髓损伤小鼠体内移植胚胎干细胞的衍生细胞可改善受损的脊髓神经功能和提高生存率.

An observation on improving functional outcome following embryonic stem cells transplantation to injured spinal cord in mouse through utilizing ethology

Objective:To observe the affection on neurological functional recovery of transplantation of embryonic stem cells(ES cell) to injured spinal cord in adult mouse.Method: Labeled XY with Lac-Z marked gene of mouse ES cell was cultured and induced to differentiate by specialized factors in vitro. ES cell-derived cells were planted into vertebral canal around injured spinal cord model. The functional outcome measurements were performed using the modified BBB score at 2, 4, and 8 weeks after transplantation.Result:An obvious decrease of mortality and a statistically significant improvement in neurological function outcome was noted in mouse of ES cell transplantation group. Conclusion: ES cell transplantation group was superior to control group in neural function recovery and mortality.