Temporal expression of AMP-activated protein kinase activation during the kainic acid-induced hippocampal cell death |
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Authors: | Ji Yeong Lee Beong Tak Jeon Hyun Joo Shin Dong Hoon Lee Jae Yoon Han Hyun Joon Kim Sang Soo Kang Gyeong Jae Cho Wan Sung Choi Gu Seob Roh |
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Affiliation: | (1) Department of Anatomy and Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University, 92 Chilam-dong, Gyeongnam, Jinju, 660-751, Republic of Korea |
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Abstract: | Kainic acid (KA)-induced seizure induces the hippocampal cell death. There are reports that AMP-activated protein kinase (AMPK), which is an important regulator of energy homeostasis of cells, has been proposed as apoptotic molecule. In this study, we investigated the altered expression of AMPK cascade in the hippocampus of mice during KA-induced hippocampal cell death. Mice were killed at 2, 6, 24 or 48 h after KA (30 mg/kg) injection. Histological evaluation of KA-treated hippocampus revealed hippocampal cell death first at 6 h and appearing prominently by 48 h after KA injection. Immunoreactivity of Ca2+/calmodulin-dependent protein kinase kinaseβ (CaMKKβ) was increased after KA treatment. In Western blot analysis, AMPK activation was increased 2 h after KA treatment. The proteins of downstream AMPK, including those of glucose transporter1 (GLUT1) and phosphorylation of Acetyl CoA Carboxylase (ACC) were increased in the hippocampus after KA treatment. These results indicate that sustained AMPK activation might be a mechanism by which KA-induced seizure causes hippocampal cell death of mice. |
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Keywords: | Kainic acid Seizure AMP-activated protein kinase Hippocampus Mice |
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