筛选大鼠心脏离心训练相关差异表达基因

目的:本研究分析离心训练对大鼠心脏基因表达的影响,并探讨离心训练提高机体+Gz耐力的分子机制。方法:应用抑制性消减杂交 (SSH)和斑点杂交技术,筛选离心训练12d后测试高耐力组 (耐受+16Gz)与未训练对照组大鼠心脏的差异表达基因。以获得的序列表达标签 (EST)为探针,分别与离心训练各组以及高耐力 (n=4)和低耐力大鼠 (耐受+12Gz,n=4)的心脏RNA进行狭线杂交,将获得的阳性EST测序,并进行同源性比较。结果:获得 3个未知下调EST,它们的表达量均在训练过程中逐渐下降。结论:离心训练可明显影响大鼠心脏某些基因的表达水平,这些基因表达水平的变化很可能与离心训练提高机体+Gz耐力的机制有关.

Differential expression of genes in the rat heart during centrifuge training

AIM: Centrifuge training can improve forward acceleration (+Gz) endurance. This study was to analyzed the gene expression of rat heart affected by centrifuge, and to research the molecular mechanism of improving +Gz endurance by centrifuge training. METHODS: Differential expressed genes between high+Gz endurance (+16Gz) rats, of test group after trained 12 d and control were screened using suppression subtractive hybridization (SSH) and dot blot hybridization. The obtained expressed sequence tags (ESTs)were used as probes to perform RNA slot hybridization with heart total RNA isolated from each gruop of centrifuge training and high+Gz endurance and low+Gz endurance (+12Gz) rats, respectively. The positive ESTs were sequenced and analyzed using BLAST(nr) at NCBI.RESULTS: Three down-regulated ESTs were obtained from heart samples, all of them are new, and their expression levels were decreasing during centrifuge training. CONCLUSION: Centrifuge training can significantly affect the special gene expressions of rat heart, and the expression changes of these genes may be ralated to the mechainism that +Gz endurance can be improved by centrifuge training.