肝癌微波消融并间质治疗后外周血T淋巴细胞的变化和意义

目的 观察肝癌间质治疗基础上联合微波消融治疗对患者外周血T淋巴细胞亚群的影响.方法 收集我院40例原发性肝癌患者,其中单纯微波消融治疗组(A组)20例,微波消融联合间质治疗组(B组)20例,分别于消融治疗前、治疗后一周应用流式细胞仪检测两组患者外周血CD3+、CD4+、CD8+、CD4+/CD8+比值.结果 治疗前两组患者外周血T淋巴细胞亚群相比较差异无显著性(P＞0.05)；A、B组手术后一周外周血CD3、CD4及CD4/CD8均较术前升高,手术前后相比较有显著性差异(P＜o.05)；A、B两组术后间比较,B组CD3、CD4及CD4/CD8值高于A组,CD8略低于B组,仅CD4有显著性差异(P＜0.05).结论 微波消融治疗原发性肝癌可使外周血T淋巴细胞亚群增加,增强细胞免疫功能,而微波消融联合间质治疗后,可使免疫功能进一步得到显著改善.

Changes of T lymphocyte cell subsets after microwave ablation combined with interstitial therapy in paitents with liver cancer

【Objective】 To study the changes of T lymphocyte cell subsets after microwave ablation combined with interstitial therapy in patients with liver cancer before and after operation.【Methods】 40 patients with liver cancer were randomly divided into A group,20 received microwave ablation alone,and B group,20 received microwave ablation combined with interstitial therapy of percutaneous lipiodol and anti-cancer agents injection intratumorly into liver cancer.Then T lymphocyte cell subsets in the peripheral blood,CD3+,CD4+,CD8+,CD4+/CD8+,were measured by flow cytometry before and one week after operation.【Results】 No difference in changes of T lymphocyte cell subsets between A and B group preoperative(P >0.05);It was higher of CD3,CD4,CD4/8 in postoperative than in preoperative of both A and B groups,there was significant difference between preoperative and postoperative in both A and B groups(P <0.05).It was higher of CD3,CD4,CD4/8 of B group than of A group in postoperative,but there was significant difference only in CD4 between A and B group postoperative(P <0.05).【Conclusion】 Microwave ablation combined with interstitial therapy for liver cancer may activate T lymphocyte cell subsets and improve immune function to much more extent than microwave ablation alone.