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| ERK1/2信号通路在角质细胞生长因子-2诱导角膜基质细胞增殖中的作用 | |
| 引用本文： | 高丽昌,李婷婷,李富勇,唐禄,李海燕,王晓杰,李校堃.ERK1/2信号通路在角质细胞生长因子-2诱导角膜基质细胞增殖中的作用[J].中国药理学通报,2011,27(10):1405-1408. |
| 作者姓名： | 高丽昌 李婷婷 李富勇 唐禄 李海燕 王晓杰 李校堃 |
| 作者单位： | 1. 吉林农业大学生物反应器与药物开发教育部工程研究中心,吉林,长春,130021;温州医学院药学院,浙江,温州,325035;吉林农业大学生命科学学院,吉林,长春,130021 2. 温州医学院药学院,浙江,温州,325035 3. 吉林农业大学生物反应器与药物开发教育部工程研究中心,吉林,长春,130021 4. 温州医学院药学院,浙江,温州,325035;吉林农业大学生物反应器与药物开发教育部工程研究中心,吉林,长春,130021 |
| 基金项目： | 国家自然科学基金资助项目，"重大新药创制"科技重大专项资助项目，吉林省科技厅资助项目，长春市科技计划资助项目，长春市企业科技特派员行动计划资助项目 |
| 摘 要： | 目的观察角质细胞生长因子(keratinocyte growthfactor-2,KGF-2)对角膜基质细胞的增殖作用及ERK1/2信号在角膜基质细胞增殖中的调控作用,探讨KGF-2对角膜基质细胞增殖的可能机制。方法体外培养角膜基质细胞,MTT法检测细胞增殖,Western blot检测磷酸化ERK1/2表达水平。结果 KGF-2在1～100 mg·L-1对角膜基质细胞有明显的促进增殖作用且呈现剂量-效应关系;100 mg·L-1 KGF-2处理角膜基质细胞,5、15、30 min后ERK1/2磷酸化表达水平明显升高,60、90、120、180 min后ERK1/2磷酸化表达水平逐渐减弱;20μmol.L-1 ERK1/2抑制剂PD98059可抑制KGF-2对角膜基质细胞的促增殖作用。结论 KGF-2激活ERK1/2信号通路,提高细胞增殖率,可被抑制剂PD98059阻断;ERK1/2信号通路调控角膜基质细胞的增殖,在角膜基质细胞损伤中发挥作用。 |
| 关 键 词： | 角质细胞生长因子-2 ERK1/2 pERK1/2 信号通路 角膜基质细胞 细胞增殖 |
Role of ERK-1/2 signaling pathway in keratinacyte growth factor-2 stimulating human keratinocyte proliferation |
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| GAO Li-chang,LI Ting-ting,LI Fu-yong,TANG Lu,LI Hai-yan,WANG Xiao-jie,Li Xiao-kun.Role of ERK-1/2 signaling pathway in keratinacyte growth factor-2 stimulating human keratinocyte proliferation[J].Chinese Pharmacological Bulletin,2011,27(10):1405-1408. | |
| Authors: | GAO Li-chang LI Ting-ting LI Fu-yong TANG Lu LI Hai-yan WANG Xiao-jie Li Xiao-kun |
| Institution: | GAO Li-chang1,2,3,LI Ting-ting1,LI Fu-yong2,TANG Lu2,LI Hai-yan1,WANG Xiao-jie2,1,Li Xiao-kun2,1(1.ME Engineering Research Center of Bioreactor and Pharmaceutical Development,Jilin Agriculture University,Changchun 130021,China,2.School of Pharmacy,Wenzhou Medical College,Wenzhou Zhejiang 325035,3.School of Life Science,China) |
| Abstract: | Aim To study the effects of extracellular signal-regulated kinase1/2(ERK1/2) on the Keratinocyte Growth Factor-2(KGF-2)-induced proliferation in human keratinocyte.Method Human keratinocytes were cultured in vitro.Cell proliferation was detected by MTT.Western-blot was used to detect the expression levels of phospho-ERK1/2.Result KGF-2(1~100 mg·L-1) significantly increased keratinocytes proliferation in a dose-dependent manner.Keratinocytes were treated with 100 mg·L-1 KGF-2 for 5min,15min and 30min,the expression levels of phosphor-ERK1/2 significantly increased and the expression levels of phosphor-ERK1/2 gradually decreased after 60,120 and 180 min.PD98059,a specific ERK1/2 inhibitor,significantly inhibited KGF-2-induced keratinocyte proliferation.Conclusion KGF-2 activates ERK1/2 signaling pathway to enhance cell proliferation rate,which can be inhibited by ERK1/2 inhibitor(PD98059).The effects of KGF-2 on keratinocyte wound healing may be related to ERK1/2signaling pathway-related role. |
| Keywords: | ERK1/2 pERK1/2 |
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