| 眼镜蛇毒细胞毒素对人肝癌细胞增殖抑制活性及作用机制的研究 |
| |
| 引用本文： | 王艳,林礼务,陈志奎,薛恩生,杨菁,俞丽云. 眼镜蛇毒细胞毒素对人肝癌细胞增殖抑制活性及作用机制的研究[J]. 中国药理学通报, 2011, 27(10): 1426-1429. DOI: 10.3969/j.issn.1001-1978.2011.10.022 |
| |
| 作者姓名： | 王艳 林礼务 陈志奎 薛恩生 杨菁 俞丽云 |
| |
| 作者单位： | 1. 福建医科大学协和医院,超声科,福建省超声医学研究所,福建,福州,350001
2. 福建医科大学协和医院,超声科,药学部,福建,福州,350001 |
| |
| 基金项目： | 福建省卫生厅青年科研课题资助项目 |
| |
| 摘 要： | 目的研究眼镜蛇毒细胞毒素(CTX)在体外诱导人肝癌BEL-7404细胞凋亡作用及其可能机制。方法不同浓度CTX作用于人肝癌BEL-7404细胞,采用噻唑蓝(MTT)比色法检测肿瘤细胞增殖情况;TUNEL法和透射电镜观察检测肿瘤细胞凋亡;并对caspase-3、-9活性、细胞色素C分布变化进行检测。结果眼镜蛇毒细胞毒素对人肝癌BEL-7404细胞具有明显的抑制增殖作用,12、24和48 h的IC50分别为2.56、1.94和1.35 mg·L-1,TUNEL法和透射电镜均观察到肿瘤细胞的凋亡;caspase-3,-9酶活性增高,Western blot检测到caspase-3,-9酶的表达增强,线粒体内Cytochrome C表达减少,而细胞质内Cytochrome C表达增强。结论 CTX首先通过线粒体细胞色素C释放到细胞质内,进一步诱导效应性caspase-3,-9酶激活,可能是其诱导人肝癌细胞凋亡而产生抗肿瘤作用的机制之一。
|
| 关 键 词： | 眼镜蛇毒细胞毒素 人肝癌细胞 凋亡 线粒体 细胞色素C caspase |
Growth inhibition activity of cytotoxin from cobra venoms on BEL-7404 cells and its mechanism |
| |
| WANG Yan,LIN Li-wu,CHEN Zhi-kui,XUE En-sheng,YANG Jing,YU Li-yun. Growth inhibition activity of cytotoxin from cobra venoms on BEL-7404 cells and its mechanism[J]. Chinese Pharmacological Bulletin, 2011, 27(10): 1426-1429. DOI: 10.3969/j.issn.1001-1978.2011.10.022 |
| |
| Authors: | WANG Yan LIN Li-wu CHEN Zhi-kui XUE En-sheng YANG Jing YU Li-yun |
| |
| Affiliation: | WANG Yan1,LIN Li-wu1,CHEN Zhi-kui1,XUE En-sheng1,YANG Jing2,YU Li-yun1(1.Fujian Provincial Ultrasonic Medicine Institute,Ultrasound Dept,2.Pharmacy Dept,Union Hospital of Fujian Medical University,Fuzhou 350001,China) |
| |
| Abstract: | Aim To investigate the effects of CTX from cobra venoms on inducing BEL-7404 apoptosis and its mechanisms.Methods The antitumor effect of CTX in BEL-7404 cells in vitro was detected by MTT;the apoptotic inducing effect was observed using TUNEL and transmission electron microscope;the enzyme activity of caspase-9 and caspase-3 was measured with biochemistry method;the expression of caspase-9,caspase-3 and cytochrome C was detected by Western blot.Results The IC50 values of CTX affected on BEL-7404 cell for 1... |
| |
| Keywords: | caspase |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
