Performance of the BD GeneOhm MRSA achromopeptidase assay for real-time PCR detection of methicillin-resistant Staphylococcus aureus in nasal specimens |
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| Authors: | Patel Parul A Ledeboer Nathan A Ginocchio Christine C Condon Susan Bouchard Stephanie Qin Peibing Karchmer Tobi Peterson Lance R |
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| Affiliation: | 1NorthShore University HealthSystem, Evanston, Illinois;2Medical College of Wisconsin, Milwaukee, Wisconsin;3NorthShore Long Island J Health System Laboratories, Lake Success, New York;4BD Diagnostics, Quebec, Quebec, Canada |
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| Abstract: | We evaluated the BD GeneOhm MRSA achromopeptidase (ACP) assay, which incorporates a new specimen preparation approach. A total of 1,216 leftover nasal samples were tested; using culture as the gold standard, the sensitivity and specificity were 92% and 94.6%, respectively. The new lysis method provides good sensitivity and simplifies specimen preparation. |
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