响应面分析法优化苦豆子多糖的醇沉工艺

目的:优化苦豆子多糖的醇沉工艺.方法:在单因素试验基础上,以多糖提取率为指标,依据响应面法原理,使用Design Expert软件的中心组合设计建立试验数学模型,考察醇沉时间、乙醇体积分数及用量对醇沉工艺的影响.采用苯酚-浓硫酸法测定苦豆子多糖含量.结果:最佳醇沉工艺条件为加4.3倍量97％乙醇醇沉26 h,苦豆子多糖提取率6.98％,与预测值(7.03％)的相对误差0.71％.结论:响应面法可有效用于优化苦豆子多糖的醇沉工艺.

Optimization of Alcohol Precipitation Technology of Polysaccharides from Sophora alopecuroides by Response Surface Methodology

Objective: To optimizate alcohol precipitation technology of polysaccharides from Sophora alopecuroides. Method: Based on single-factor test,with yield of polysaccharides as index,precipitation time,the concentration and adding multiples of ethanol was optimized according to mathematic model built by central composite design in Design Expert software on principle of response surface methodology.The content of polysaccharides from S. alopecuroides was determined by phenol-concentrated sulfuric acid method. Result: Optimum alcohol precipitation technology was as following:precipitation time of 26 h,the concentration of ethanol 97%,adding multiples of ethanol 4.3 times.Under these conditions,yield polysaccharides from S. alopecuroides was 6.98% while the predicted value was 7.03%,their relative error was 0.71%. Conclusion: Response surface methodology was effective to optimize alcohol precipitation technology of polysaccharides from S. alopecuroides.