脊髓3/4横断伤动物模型的建立及其电生理评价

背景:建立稳定、标准的脊髓损伤动物模型是研究脊髓损伤修复的前提.目的:建立一种实用、标准、可靠的急性大鼠脊髓损伤模型.方法:将60只成年雌性Wistar大鼠随机均分成两组.脊髓损伤组:打开T8胸椎对应脊髓,剪开硬膜,用特殊设计的眼科维纳斯剪横跨脊髓背侧中线剪断脊髓的后3/4,深度1.5 mm.1 h后行感觉诱发电位和运动诱发电位检查.对照组:只打开椎板,剪开硬膜,暴露脊髓进行假手术.结果与结论:造模成功率为100%.对照组造模后1周功能恢复接近正常;脊髓损伤组造模后第2周开始恢复,到第4周基本停止,最终运动功能评分未超过10分,两组比较差异有显著性意义.脊髓损伤组可以明显看到感觉诱发电位与运动诱发电位的波幅值急剧降低,且潜伏期明显延长,差异有显著性意义(P < 0.01).说明3/4横断伤急性大鼠脊髓损伤模型制作法操作简便、重复性好,是研究脊髓再生修复的理想模型.

Establishment and electrophysiology evaluation of an animal model of 3/4 crosscut spinal cord injury

BACKGROUND: Establishing a stable and standard animal model of spinal cord injury is a precondition to investigate the repair of spinal cord injury. OBJECTIVE: To establish a practical, standard and dependable model of acute spinal cord injury in rats. METHODS: A total of 60 female Wistar rats were randomly assigned into two groups, spinal cord injury group and control group. The spinal cord corresponding to T8 thoracic vertebrae of spinal cord injury rats were open. The dura mater was sheared opening. The posterior 3/4 of the spinal cord was cut across the spinal dorsal midline by a specially designed Venus eye scissors, 1.5 mm in depth. The sensory evoked potentials and motor evoked potentials were examined in an hour. As for the control rats, the vertebral lamina and dura mater were opened. The spinal cord was exposed for sham operation. RESULTS AND CONCLUSION: The success rate of model construction was 100%. The function recovery of control group was close to normal in the 1st week after model establishment. The function recovery of spinal cord injury group started in the 2nd week after model establishment and virtually stopped in the 4th week. The final motor-function grades were no more than 10. There was significant difference between the two groups. The wave amplitude of sensory evoked potentials and motor evoked potentials in spinal cord injury group decreased sharply and significantly, the latency extended significantly (P < 0.01). These findings indicate that the establishment of rat acute spinal cord injury model of 3/4 traumatic transection is easy and reproducible. It is an ideal model for the investigation of spinal regenerative repair.