丝素蛋白/壳聚糖复合支架材料的细胞相容性

背景:大量研究表明丝素蛋白、壳聚糖为天然高分子材料,具有良好的细胞生物相容性.目的:探讨丝素蛋白/壳聚糖复合支架材料与诱导的兔骨髓间充质干细胞的生物相容性.方法:将兔骨髓间充质干细胞分离培养、诱导后,与丝素蛋白/壳聚糖三维支架材料体外共培养,以材料的细胞毒性、细胞增殖活力、材料细胞黏附率及扫描电镜等检测评价材料的细胞相容性.结果与结论:经诱导后的骨髓间充质干细胞在支架材料上黏附、生长良好,保持正常的分裂增殖速度;随时间的增加,细胞黏附率增加,材料组较对照组黏附率强,差异有显著性意义(P<0.05).扫描电镜观察发现细胞接种48 h 后细胞生长良好,与支架黏附紧密,增殖分裂活跃.说明丝素蛋白/壳聚糖三维支架材料具有良好的细胞相容性.

Cellular biocompatibility of silk fibroin/chitosan composite scaffolds

BACKGROUND:Many studies show that silk fibroin(SF) and chitosan(CS) are natural polymer materials that have good biocompatibility.OBJECTIVE:To discuss the biocompatibility of induced rabbit bone marrow mesenchymal stem cells(BMSCs) with SF/CS composite scaffolds.METHODS:Rabbit BMSCs were isolated,cultured and induced,and then cocultured with three-dimensional SF/CS scaffolds in vitro.Cell toxicity of scaffolds,cell viability,cell adhesion rate were determined to evaluate cellular biocompatibility of the materials as well as scanning electron microscope(SEM) observation.RESULTS AND CONCLUSION:After being induced,the rabbit BMSCs adhered and grew well on the scaffolds to keep normal division and proliferation.As time went,the cell adhesion rate increased and the scaffolds group had a stronger adhesion rate than the control group,and the difference had statistic meaning(P < 0.05).The SEM observation found that the cells grew well after 48 hours,which were closely adhered to scaffolds as well as divided and proliferated actively.The above findings indicate that the three-dimensional SF/CS scaffolds have a better cellular biocompatibility.