长期培养对基质细胞衍生因子1/CXCR4介导间充质干细胞迁移的影响

背景:人外源性的间充质干细胞能特异性地向损伤部位迁移,参与多种组织的损伤修复,然而其定向迁移的机制尚不清楚.目的:观察基质细胞衍生因子1对骨髓间充质干细胞定向迁移的影响.方法:体外培养不同个体的骨髓间充质干细胞,培养至第10代,检测细胞衰老状态.RT-PCR和细胞免疫荧光检测骨髓间充质干细胞CXCR4受体表达情况;体外迁移体系(Transwell)检测基质细胞衍生因子1对骨髓间充质干细胞迁移的影响.结果与结论:骨髓间充质干细胞在体外长期增殖后生长逐渐缓慢,在第6,7代衰老细胞增多,其CXCR4受体表达减少.基质细胞衍生因子1对骨髓间充质干细胞的趋化作用呈剂量依赖性.

Stromal cell-derived factor-1/CXCR4 effects on ex vivo expanded bone marrow mesenchymal stem cells migration

BACKGROUND: Exogenous human mesenchymal stem cells (MSCs) possess an ability to selectively migrate to the site of injury, and may further repair various damaged tissue. The mechanisms of directional migration remain unclear. OBJECTIVE: To examine the expression of the CXCR4 receptor in MSCs after long-term culture ex vivo, and to investigate the effects of stromal cell derived factor-1 (SDF-1) at various concentrations on MSCs migration. METHODS: MSCs were cultured ex vivo to passage 10, and then cell senescence was determined by β-galactosidase staining. The expression of CXCR4 receptor in MSCs was examined by RT-PCR and immunofluorescence staining. Transwell system was used to detect in vitro migration capacity of MSCs towards SDF-1. RESULTS AND CONCLUSION: Proliferation gradually decreased in all samples in the course of long-term culture ex vivo, and became restricted to senescent stages at passage 6 or 7. The expression of CXCR4 receptor was gradually lost with culture expansion. SDF-1 induced the migration of MSCs in a dose-dependent manner.