骨髓间充质干细胞移植促进糖尿病大鼠后肢缺血血管的新生

背景:骨髓间充质干细胞是否是促进下肢缺血血管新生的主要种子细胞来源呢?目的:探讨骨髓间充质干细胞肌肉注射移植诱导大鼠糖尿病后肢缺血的血管新生证据。方法:采用腹腔注射链脲佐菌素(STZ)结合双侧股动脉结扎制备雄性SD大鼠糖尿病后肢缺血模型。自股动脉离断处起分10个点将BrdU标记BMSCs悬液注射入模型大鼠左后肢股直肌和腓肠肌中;右后肢采用等量生理盐水注射作为对照。结果与结论:移植2周后模型大鼠双后肢可见血管显影,6周后血管更为丰富,左侧均高于右侧。移植后2周,左后肢股直肌与腓肠肌的毛细血管密度分别高于右后肢的密度(P<0.05);BrdU标记阳性移植细胞参与了左后肢血管壁构成。表明局部注射移植骨髓间充质干细胞可改善糖尿病后肢缺血大鼠的血供,并参与小血管重建,有助于糖尿病后肢缺血的血管新生。

Angiogenesis in lower limb ischemia of diabetic rats after bone mesenchymal stem cells transplantation

BACKGROUND: Whether bone marrow mesenchymal stem cells (BMSCs) is the major source of seed cells to promote angiogenesis in lower limb ischemia. OBJECTIVE: To investigate the angiogenesis evidence in lower limb ischemia of diabetic rats after intramuscular injection of BMSCs. METHODS: A rat diabetic ischemic lower limb model was made by intraperitoneal injection of streptozotocin plus ligation of the bilateral femoral arteries. BrdU-labeled BMSCs were injected into 10 points of the left lower limb ischemic muscles such as rectus femoris and gastrocnemius beginning at ligation site. The same muscles of right lower limb were injected with equivalent normal saline as control. RESULTS AND CONCLUSION: Digital subtraction angiography imaging analysis showed that vasculature of bilateral legs in model rats could be observed at 2 weeks post-transplantation, and was obviously increased at 6 weeks after implantation of BMSCs, the vasculature in left leg was always higher than that in the right. At 2 weeks after transplantation, capillary densities of rectus femoris and gastrocnemius in left leg were higher than right leg (P < 0.05), and some implanted BMSCs with positive BrdU in vascular wall, which might have been incorporated into the vasculature. It is indicated that transplantation of BMSCs by local injection can improve blood supply and contribute to angiogenesis in lower limb ischemia of diabetic rats.