间断直立位下兔椎间盘退变模型的影像及病理表现

背景:已有大量的动物模型用于研究椎间盘退变,但是尚缺乏一种符合人类椎间盘退变规律的动物模型。目的:探讨直立位下构建椎间盘退变模型的可行性及造模的简便、易行性。方法:新西兰大白兔42只,随机数字表法均分为实验组和对照组。将兔子固定在PVC管中,实验组将PVC管直立,对照组将PVC管平放。结果与结论:4周时实验组和对照组间椎间盘高度指数、MRI及纤维环改变差异无显著性意义(P>0.05),髓核PAS染色后灰度值明显增加(P<0.05)。8,12周实验组和对照组椎间盘高度指数相比差异有显著性意义,退变椎间盘T2加权像信号明显降低,苏木精-伊红染色显示纤维环排列紊乱,PAS染色显示退变椎间盘髓核的灰度值明显变大(P<0.05)。说明通过强迫直立位下因自身身体重力负荷对椎间盘产生影响可以成功建立椎间盘退变模型。

Pathology and imaging performance of a rabbit intervertebral disc degeneration model induced by the intermittent upright position

BACKGROUND: A great number of animal models were used to study intervertebral disc degeneration, but there is still lack of a consistent with human intervertebral disc degeneration in animal models. OBJECTIVE: To investigate the feasibility of establishing a intervertebral disc degeneration model with upright position and a model construction with convenience and simple. METHODS: Totally 42 New Zealand white rabbits were randomly divided into experimental group and control group. The rabbits were fixed in polyvinyl chloride (PVC) pipes. The PVC pipes were upright in the experimental group and the PVC pipes were flat in the control group. RESULTS AND CONCLUSION: At 4 weeks after modeling, there was no significant difference of the disc height index (DHI), magnatic resonance imaging and annulus change between the experimental group and the control group (P > 0.05). PAS staining of nucleus, the gray value was increased significantly (P < 0.05). At 8, 12 weeks after modeling, there was significant difference of the DHI in the two groups, which has a significant decreased in degenerative disc T2-weighted signal. Hematoxylin-eosin staining of annulus was disordered arrangement. PAS staining of degenerative disc intervertebral nucleus showed that gray value was significantly larger (P < 0.05). It is indicated that the method of upright position can successfully establishment of disc degeneration model by their own gravity load on the intervertebral disc.