| 间充质干细胞移植抑制小鼠急性移植物抗宿主病的实验研究(英文) |
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| 引用本文： | 李红,郭子宽,李秀森,江小霞,朱恒,要辉宇,王晓燕,苏永丰,廖丽,刘元林,吴英,张毅,毛宁. 间充质干细胞移植抑制小鼠急性移植物抗宿主病的实验研究(英文)[J]. 组织工程与重建外科, 2010, 6(3): 121-127 |
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| 作者姓名： | 李红 郭子宽 李秀森 江小霞 朱恒 要辉宇 王晓燕 苏永丰 廖丽 刘元林 吴英 张毅 毛宁 |
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| 作者单位： | 李红,刘元林,吴英,张毅,毛宁,李秀森,江小霞,朱恒,要辉宇,王晓燕,苏永丰(军事医学科学院基础医学研究所细胞生物学研究室,北京市,100850);郭子宽(军事医学科学院放射与辐射医学研究所实验血液学研究室,北京市,100850);廖丽(军事医学科学院307医院造血干细胞移植中心,北京市,100071) |
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| 基金项目： | 国家重点基础发展研究规划(973)项目，国家高科技研究发展计划(863)项目(30900568 |
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| 摘 要： | 目的研究间充质干细胞的体内免疫调节活性。方法将C57/BL脾脏细胞输注入受亚致死量照射的BALB/c小鼠,建立急性移植物抗宿主病模型,受体小鼠或同时接受密质骨骨髓来源的间充质干细胞,流式细胞技术检测脾细胞表面表达的活性分子。结果模型经输注间充质干细胞后,鼠脾脏CD11b+细胞表达的CD86和CD69减少,提示抗原呈递细胞的成熟度下降。此外,作为一种效应T细胞早期活化和发育的标记,CD3+CD69+细胞/CD3+细胞的比值降低,导致脾内CD3+细胞的绝对和相对数目减少。但是,在同系脾细胞输注模型中,CD11b+细胞表面的CD69和MHCⅡ表达以及CD3+细胞表面的CD69不受影响。结论 MSCs可以分3个阶段抑制急性移植物抗宿主病,有望成为急性移植物抗宿主病的有效治疗手段,但仍需进行深入研究。
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| 关 键 词： | 急性移植物抗宿主病 抗原呈递细胞 免疫调节 体内间充质干细胞 T淋巴细胞 |
Experimental Research on Inhibition of Acute Graft-versus -Host Disease by Transplanted Mesenchymal Stem Cells in Murine Model |
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| LI Hong,GUO Zikuan,LI Xiusen,JIANG Xiaoxia,ZHU Heng,YAO Huiyu,WANG Xiaoyan,SU Yongfeng,LIAO Li,LIU Yuanlin,WU Ying,ZHANG Yi,MAO Ning. Experimental Research on Inhibition of Acute Graft-versus -Host Disease by Transplanted Mesenchymal Stem Cells in Murine Model[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2010, 6(3): 121-127 |
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| Authors: | LI Hong GUO Zikuan LI Xiusen JIANG Xiaoxia ZHU Heng YAO Huiyu WANG Xiaoyan SU Yongfeng LIAO Li LIU Yuanlin WU Ying ZHANG Yi MAO Ning |
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| Affiliation: | LI Hong1,GUO Zikuan2,LI Xiusen1,JIANG Xiaoxia1,ZHU Heng1,YAO Huiyu1,WANG Xiaoyan1,SU Yongfeng1,LIAO Li3,LIU Yuanlin1,WU Ying1,ZHANG Yi1,MAO Ning1 1 Department of Cell Biology,Beijing Institute of Basic Medical Sciences,Beijing 100850,China,2 Department of Experimental Haematology,Institute of Radiation Medicine,3 Department of Hematopoietic Stem Cell Transplantation,Affiliated Hospital to Academy of Military Medical Sciences,Beijing 100071,China. |
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| Abstract: | Objective To investigate the in vivo immunoregulatory activities of mesenchymal stem cells (MSCs). Methods A murine aGvHD model was developed by transfusion of C57/BL splenocytes, with or without compact bone-derived MSCs, into sublethally irradiated BALB/c mice. The phenotypic features of the splenocytes were analyzed by flow cytometry after transplantation. Results MSCs infusion decreased the expression of CD86 and CD69 molecules on splenic CD11b+ cells of aGvHD mice, suggesting of the decreased maturatio... |
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| Keywords: | Acute Graft-versus-Host Disease Antigen presenting cells Immunoregulation In vivo mesenchymal stem cells T lymphocyte |
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