|
|||||
|
|
Simultaneous Visualization of Vasopressin and Oxytocin mRNA- Containing Neurons in the Hypothalamus Using Non-Radioactive in situ Hybridization Histochemistry |
|
| Authors: | Yosuke Ichimiya Piers C. Emson Chris Christodoulou Michael J. Gait Jerry L. Ruth |
| Affiliation: | MRC Group, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4AT, UK.;MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.;** Molecular Biosystem Inc., 10030 Barnes Canyon Road, San Diego, California 92121, USA. |
| Abstract: | In situ hybridization histochemistry (ISHH) using synthetic oligodeoxynucleotide probes has been used to demonstrate the sites of expression of mRNA for vasopressin (AVP) and oxytocin (OXT) in the rat brain. ISHH was performed with two types of non-radioactive probes, labelled with either alkaline phosphatase or 5'-biotin. Simultaneous detection of the mRNAs for both AVP and OXT was achieved using an alkaline phosphatase substrate for the AVP probe and an anti-biotin monoclonal (mouse) antibody for the OXT probe. These probes revealed two non-overlapping populations of AVP and OXT neurons on the same section. |
| Keywords: | in situ hybridization histochemistry alkaline phosphatase biotin vasopressin oxytocin |