免疫毒素192-IgG-saporin致痴呆动物基底前脑一氧化氮合酶阳性神经元的变化

目的:建立侧脑室注射免疫毒素192-IgG-saporin致痴呆动物模型,观察其学习记忆能力和基底前脑一氧化氮合酶(NOS)阳性神经元的变化。方法:侧脑室注射免疫毒素192-IgG-saporin,行Y迷宫检测,采用NADPH-d组织化学法染色,结合图像分析技术观察大鼠基底前脑NOS阳性神经元数目和形态学参数的变化。结果:模型组大鼠的学习、记忆能力较正常组明显下降。模型组基底前脑内侧隔核(MS)和斜角带垂直支(VDB)NOS阳性神经元数分别减少到正常组的21.62%和17.70%;细胞周长和面积降低,灰度值升高,均有显著性差异。结论:应用192-IgG-saporin免疫毒素可使基底前脑NOS阳性神经元明显减少。

Changes in NOS-positive neurons of basal forebrain in rat Alzheimer's model induced by immunotoxin 192-IgG-saporin

Objective:To establish an animal model of Alzheimer's disease in rats and observe changes in learning and memory abilities and in NOS-positive neurons of the basal forebrain.Methods:192-IgG-saporin was injected into the lateral ventricle of SD rats.Learning and memory abilities were tested by Y-maze.Morphologic data and percentages of NOS-positive neurons were measured in the basal forebrain by using NADPH-d histochemical method combined with image analysis.Results:The learning and memory abilities in the model group were sharply decreased,compared with the normal group.21.62 %and 17.70 %of NOS-positive neurons respectively survived in medial septum(MS)and vertical diagonal band(VDB)of the basal forebrain in the model group.The area and perimeter of NOS-positive neu- rons in MS/VDB of the model group were significantly decreased.The gray value in MS/VDB of the model group was markedly increased.Conclusion:The number of NOS-positive neurons significantly decreases in the basal forebrain u- sing immunotoxin 192-IgG-saporin in rat.