| 突触后密度蛋白95在大鼠脊髓损伤后的表达变化 |
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| 引用本文： | 沈爱国,高尚锋,程纯,赵剑,陈梦玲,牛淑琼,李欣,郭志琴. 突触后密度蛋白95在大鼠脊髓损伤后的表达变化[J]. 解剖学杂志, 2007, 30(2): 160-164 |
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| 作者姓名： | 沈爱国 高尚锋 程纯 赵剑 陈梦玲 牛淑琼 李欣 郭志琴 |
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| 作者单位： | 南通大学江苏省神经再生重点实验室,南通,226001;南通大学基础医学院微生物与免疫学教研室,南通,226001;南通大学基础医学院微生物与免疫学教研室,南通,226001;南通大学附属医院骨科,南通,226001;南通大学江苏省神经再生重点实验室,南通,226001 |
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| 基金项目： | 国家自然科学基金(30300099)，江苏省自然科学基金(BK2003035)，江苏省高校自然科学研究项目(03KJB180109、04KJB320114)，江苏省社会发展科技指导性计划项目(BS2004526)，江苏省“六大人才高峰”二批资助项目 |
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| 摘 要： | 目的:探讨突触后密度蛋白95(PSD-95)在脊髓损伤(SCI)后的表达变化以及定位情况。方法:使用改良Allen's法建立大鼠急性脊髓损伤模型。采用实时定量PCR和Western印迹法测定损伤后各时间段PSD-95 mRNA及其蛋白水平在脊髓中的表达变化。采用免疫荧光双标方法显示PSD-95在正常脊髓中的分布以及损伤后的定位改变。结果:PSD-95 mRNA及其蛋白水平在SCI后呈现逐渐下降的趋势,mRNA在5 d降到最低水平,蛋白水平在7 d最低。PSD-95与神经型一氧化氮合酶(nNOS)在SCI后8 h存在着共定位关系,但在SCI后7d,PSD-95除与nNOS部分共定位之外,还高表达于损伤局部活化中性粒细胞和巨噬细胞。结论:SCI后PSD-95在基因和蛋白水平呈现明显的时相变化,并且损伤后高表达在活化的炎症细胞,提示PSD-95参与了脊髓继发性损伤过程。
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| 关 键 词： | 突触后密度蛋白95 脊髓损伤 神经型一氧化氮合酶 |
| 修稿时间： | 2006-10-082006-12-11 |
Expression of postsynaptic density protein - 95 in injuried spinal cord of rats |
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| Shen Aiguo,Gao Shangfeng,Cheng Chun,Zhao Jian,Chen Mengling,Niu Shuqiong,Li Xin,Guo Zhiqin. Expression of postsynaptic density protein - 95 in injuried spinal cord of rats[J]. Chinese Journal of Anatomy, 2007, 30(2): 160-164 |
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| Authors: | Shen Aiguo Gao Shangfeng Cheng Chun Zhao Jian Chen Mengling Niu Shuqiong Li Xin Guo Zhiqin |
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| Affiliation: | 1 Jiangsu Province Key Laboratory of Neural Regeneration of Nantong University ; 2 Department of Microbiology and Immunology, Basical Medical College of Nantong University ; 3 Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong 226001, China |
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| Abstract: | Objective:To study the expression and distribution of postsynaptic density protein-95 (PSD-95) after spi- nal cord injury (SCI).Methods:With improved Allen's method,rat acute SCI models were established.Real Time- PCR and Western blotting were used to detect the different expression of PSD-95 mRNA and protein levels after SCI. With the method of immunofluorescenee double staining,the distribution in normal spinal cord and the localization changes of PSD-95 after SCI were investigated.Results:The expression of PSD-95 mRNA and protein gradually de- creased after SCI.The minimal mRNA appeared at 5 d,whereas the minimal protein existed at 7 d.PSD-95 was co-lo- calized with neuronal nitric oxide synthase (nNOS) in the impaired spinal cord at 8 h;at 7 d after SCI,PSD-95 was expressed in the inflammatory cells of lesion site except that it was associated with nNOS.Conclusion:The expression of PSD-95 and its mRNA varies significantly after SCI,and its distribution in the injuried spinal cord is different from that in the normal,which indicate that PSD-95 may participate in the following response after SCI. |
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| Keywords: | postsynaptic density protein-95 spinal cord injury neuronal nitric oxide synthase |
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