复发性自然流产患者绒毛及蜕膜组织中白细胞介素8的表达

目的:研究复发性自然流产患者绒毛及蜕膜组织中白细胞介素8(interleukin-8,IL-8)的表达。方法:应用免疫组化法及图像分析技术,对30例复发性自然流产患者绒毛、蜕膜组织中IL-8的蛋白表达进行定位及半定量分析;H- E染色后光镜下观察绒毛及蜕膜组织的形态学变化。结果:在绒毛组织中,IL-8蛋白定位于绒毛上皮细胞的细胞质内,且病例组的表达明显高于对照组;在蜕膜组织中,病例组蜕膜细胞的胞质内可见IL-8蛋白表达,且高于对照组;H- E染色可见病例组绒毛组织的滋养层变薄,细胞变性甚至坏死、嗜酸性增强,绒毛中轴纤维化程度增强;蜕膜组织中蜕膜细胞失去细胞间连接,部分蜕膜细胞解体、核消失。结论:病例组绒毛及蜕膜组织中IL-8蛋白表达的增强与复发性自然流产有关,IL-8可能参与复发性自然流产的病理过程。

Expression of interleukin-8 in villi and decidua of patients with early recurrent spontaneous abortion

Objective:To study the expression of interleukin-8(IL-8)in villi and decidua of patients with recurrent spontaneous abortion(RSA).Methods:The localization and quantitative expressions of IL-8 in villi and decidua of 30 RSA patients were shown and measured by immunohistochemistry method and image analysis;The morphological changes of villi and decidua in RSA were observed with light microscope by hematoxylin-eosin staining.Results:IL-8 protein was located in the epithelium of villi.The expression of IL-8 was significantly increased in RSA group than that in the control group.Positive IL-8 cells was shown in decidua of RSA group,and its IL-8 level was higher than that in the control group.By hematoxylin-eosin staining,trophoblastic layer was found to get thinner,cells of trophoblastic layer denatured or neerotized,and turned acidophily,and the fibration of villous axis increased in RSA;decidual cells lost connection,a part of decidual cells appeared cytoclasis and turned more acidophilic,and nuclei disappeared in RSA.Conclusion:The higher expression of IL-8 in villi and decidua was releated to RSA.IL-8 may take part in the pathologic process of RSA.