| 微量接触类生物检材的游离 DNA 问题分析 |
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| 作者姓名： | 董会 魏丽 张涛 王晶 李彩霞 刘超 |
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| 作者单位： | 1.公安部物证鉴定中心法医遗传学公安部重点实验室，北京1000382.广州市刑事科学技术研究所，广东 广州 5100303.南方医科大学，广东 广州 5105154.公安部物证鉴定中心北京市现场物证检验工程技术研究中心，北京 1000385.山西医科大学，山西 太原 030000 |
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| 摘 要： | 目的探究接触类生物检材的游离DNA问题，游离DNA和细胞内DNA含量的比例问题，以及游离DNA在法医检验中的影响。方法对手部、面部和足部的皮肤及口腔粘膜进行模拟微量接触类检材的制备，对模拟检材进行浸泡，对游离DNA进行定量和STR扩增，对剩余检材使用常规程序进行DNA提取、定量和STR扩增，并对结果进行分析和讨论。 结果实验观察到，78.55%的样品检测到了游离DNA，其中，手部模拟检材的游离DNA检测率为82.1%，脸部模拟检材的游离DNA检测率为82.1%，口腔模拟检材的游离DNA检测率为96.4%，足部模拟检材的游离DNA检测率为53.6%，微量接触类检材中游离DNA量占DNA总量的12.9%。 结论接触类生物检材中提取到的DNA，除了包括细胞内DNA，还存在一定比例的细胞外游离DNA，这为微量接触类检材的DNA有效提取和检验提供了新的理论依据。
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| 关 键 词： | 法医物证学 游离DNA 微量接触类检材 短串联重复序列 |
| 收稿时间： | 2015-03-21 |
Explorations of cell-free DNA on trace touch DNA samples |
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| Authors: | Hui DONG Li WEL Tao ZHANG Jing WANG Caixia LI Chao LIU |
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| Institution: | 1.Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China2.Guangzhou Forensic, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou 510030, China3.Southern Medical University, Guangzhou 510515, China4.Beijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science, Beijing 100038, China5.Shanxi Medical University, Taiyuan 030000, China |
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| Abstract: | Objective To investigate cell-free DNA on trace touch DNA samples and the amount and proportion of the cell free DNA and the intracellular DNA and the influence of cell-free DNA in forensic analysis. Methods To manufacture mock exhibits and then the DNA extracted from the samples was quantified using standard manufacture's procedures, and the results were analyzed and discussed. Results This experiment shows that the cell free DNA has been detected in 78.55% of the samples. The detection rates in hand touch DNA samples, face touch DNA samples, foot touch DNA samples and mouth-swab respectively are 82.1%,82.1%,53.6% and 92.4%. The amount of cell free DNA is 12.9% of the amount of total DNA in trace/touch DNA samples. Conclusion The cell free DNA did exist extracellular, and the amount of the cell free DNA occupy a proportion in the exhibits of touch DNA. This study showed a new theory of the question of cell free DNAof touch DNA. |
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