p53、p63及p73在皮肤血管瘤组织中的表达

目的　为探讨p5 3p6 3及p73蛋白在皮肤血管瘤组织中的表达及意义。方法　采用免疫组织化学S P法和图像分析技术检测 4 0例血管瘤组织和 2 0例正常皮肤组织中p5 3、p6 3及p73基因的表达。结果　在增生期血管瘤、退化期血管瘤和正常皮肤组之间 ,p73蛋白免疫组化阳性反应颗粒的平均光密度分别为 6 4 0 8± 2 15 1、1 0 73± 0 5 16和 0 95 3± 0 12 0。p6 3蛋白免疫组化阳性反应颗粒的平均光密度分别为 8 2 71± 1 95 3、0 92 3± 0 191和 0 92 0± 0 187。p5 3蛋白免疫组化阳性反应颗粒的平均光密度分别为 7 2 4 0± 1 874、0 934± 0 187和 0 92 3± 0 16 5。增生期血管瘤与退化期血管瘤、正常皮肤组分别组比 ,p73、p6 3及p5 3阳性表达的差异均有高度显著性差异 (P <0 0 0 1) ,消退期血管瘤与正常皮肤组之间 ,p73、p6 3及p5 3阳性表达差异无显著性 (P >0 0 5 )。结论　在增生期血管瘤组织中p73、p6 3及p5 3呈高表达 ,促进了内皮细胞的增殖 ,是导致血管瘤发生、发展的主要因素

Expression of p73,p53 and p63 in skin hemangioma

Objective To investigate the quantitative expression of p73,p53 and p63 protein and its significance in skin hemangioma.Methods The immunohistochemical and image analysis technique were used to detect p73,p53 and p63 protein expression in 40 hemangioma and 20 normal skin tissue.Results average optical density of p73 protein positive immunostaining was 6 408±2 151,1 073±0 516 and 0 953±0 120,average optical density of p63 protein positive immunostaining was 8 271±1 953,0 923±0 191 and 0 920±0 187,average optical density of p53 protein positive immunostaining was 7 240±1 874,0 934±0 187 and 0 923±0 165 respectively,among proliferative hemangioma,involutional stage of hemangioma and normal skin tissue.Proliferative hemangioma and involutional stage of hemangioma,normal skin tissue respectively,there were significant differences of p73,p63 and p53 postiive expression( P <0 001),no statistical significance of p73,p63 and p53 expression was observed between involutional stage of hemangioma and normal skin tissue( P >0 05).Conclusion p73,p63 and p53 expresses highly in tissue of proliferative hemangioma,it promotes proliferation of endothelial cell,it is the main factor that leads to the occurrence and development of hemangioma.