|
|||||
|
|
Envelope and Long Terminal Repeat Sequences of an Infectious Murine Leukemia Virus from a Human SCLC Cell Line: Implications for Gene Transfer |
|
| Authors: | Antoine Marianne Wegmann Barbara Kiefer Paul |
| Institution: | 1.Ruhr-Universita¨t Bochum, Medizinische Fakulta¨t, Institut fu¨r Hygiene und Mikrobiologie, Abteilung fu¨r Med. Mikrobiol. und Virologie, Universita¨tsstra?e 150, D-44780 Bochum, Geba¨ude MA 6/130, Germany ;2.Philipps Universita¨t Marburg, Zentrum fu¨r Innere Medizin, Abteilung fu¨r Ha¨matologie/Onkologie, Baldingerstra?e, D-3550, Marburg, Germany ; |
| Abstract: | Development of methods for gene transfer into specific cell types or tissues is important for experimental research as well as clinical therapeutical approaches. We report here the cloning and characterization of the envelope (env) gene and the U3 region of a retrovirus from an infected human Small Cell Lung Cancer (SCLC) cell line. The replication of this murine retrovirus is also fully supported by other lung cancer cell lines of different histological origin. We present evidence that a long terminal repeat (LTR)-β-galactosidase (β-Gal) reporter construct performed as well as an analogous cytomegalovirus (CMV) promoter β-Gal construct in the human lung epithelial cell line A549 and in the human larynx carcinoma cell line HEp2. This revised version was published online in July 2006 with corrections to the Cover Date. |
| Keywords: | xenotropic endogenous MuLV gene transfer Bxv-1 |
| 本文献已被 SpringerLink 等数据库收录！ | |