GC-MS技术分析红车轴草和白车轴草种子挥发性成分

目的:分析与比较红车轴草和白车轴草种子挥发性成分的差异。方法:采用顶空固相微萃取(HS-SPME)法萃取挥发性成分,结合气相色谱质谱联用(GC-MS)技术分析其组成,面积归一化法计算各成分的相对含量。结果:红车轴草种子中鉴定出46种化合物,挥发性成分总峰面积为3. 48×109。其中相对质量分数较高的化合物为1-辛烯-3-醇(30. 24%),正己醇(11. 94%),2,2,4,6,6-五甲基庚烷(11. 00%),壬醛(7. 74%),辛醛(6. 79%)和己酸(6. 04%)。白车轴草种子中鉴定出44个化合物,挥发性成分总峰面积为6. 37×109。其中相对质量分数较高的化合物为1-辛烯-3-醇(42. 98%),十七烷(14. 47%),3-辛醇(9. 42%),己酸乙烯酯(8. 82%)和5-甲基-4,6-二羟基嘧啶(6. 59%)。红车轴草和白车轴草种子共有20种成分相同,但各成分的含量具有较大差异。1-辛烯-3-醇为共有成分中含量最高的物质。红车轴草种子有26种特有成分,占挥发性组分的54. 85%。白车轴草种子有24种特有成分,占挥发性组分的46. 79%。结论:红车轴草和白车轴草种子中含有较为丰富的挥发性成分,两品种之间挥发性成分的种类、峰面积和相对含量具有较大差异。

Analysis and Comparison of Volatile Components in Trifolium pratense and Trifolium repens Seed by GC-MS

Objective: To analyze and compare the difference of volatile components between Trifolium pratense and T. repens seeds.Method: The volatile components were extracted by the headspace solid phase microextraction (HS-SPME) method and analyzed by gas chromatography-mass spectrometry (GC-MS),and the relative contents of the components were calculated by the area normalization method.Result: Fourty six compounds were identified in the seeds of T. pratense,with a total peak area of 3.48×10⁹ for the volatile components. The compounds with high content in this species were 1-octen-3-ol (30.24%),1-hexanol (11.94%),heptane,2,2,4,6,6-pentamethyl (11.00%),nonanal (7.74%),octanal (6.79%) and hexanoic acid (6.04%). 44 compounds were identified from the seeds of T. repens,with a total peak area of 6.37×10⁹ for the volatile components. The compounds with high content in this species were 1-octen-3-ol (42.98%),heptadecane (14.47%),3-octanol (9.42%),n-caproic acid vinyl ester (8.82%) and pyrimidine-4,6-diol,5-methyl (6.59%). 20 components were commonly shared in the seeds of T. pratense and T. repens,but the content of each component was quite different. 1-octen-3-ol had the highest content in the common components. T. pratense seeds had 26 unique components,accounting for 54.85%of volatile components. T. repens seeds had 24 unique components,accounting for 46.79%of volatile components.Conclusion: The seeds of both T. pratense and T. repens have rich volatile components,but there are great difference in the variety,peak area and relative content of the volatile components between these two varieties