基于UPLC-Q-TOF-MS技术的黑骨藤血清药物化学分析

目的:对黑骨藤的血清药物化学进行研究,探讨黑骨藤提取物在大鼠体内的药效物质基础。方法:采用UPLCQ-TOF-MS技术,以及Data Analysis和Metabolite Detect等软件分析,通过比对黑骨藤提取物、含药血清、空白血清指纹图谱以及空白血清与含药血清的差异图谱,根据质谱所提供的保留时间、精确相对分子质量以及与对照品的比较,判定口服黑骨藤提取物后在大鼠体内的入血成分。结果:口服黑骨藤提取物后,从血清中检测出17个入血成分,其中10个为原型成分,7个为代谢产物。鉴定了其中7个原型吸收入血成分,依次为5-O-咖啡酰基奎宁酸,4-O-咖啡酰基奎宁酸,3-O-咖啡酰基奎宁酸,3,4-O-二咖啡酰基奎宁酸,3,5-O-二咖啡酰基奎宁酸,4,5-O-二咖啡酰基奎宁酸、杠柳毒苷。结论:这些入血成分可能是黑骨藤在体内直接作用的物质,有助于阐明其药效物质基础和作用机制。

Serum Pharmacochemical Analysis of Periploca forrestii Rhizomes Based on UPLC-Q-TOF-MS

Objective:To study the serum pharmacochemistry of Periploca forrestii rhizomes,and to investigate the pharmacological material basis of extract of P. forrestii rhizomes in rats. Method:Rapid identification of constituents absorbed into blood was carried out by UPLC-Q-TOF-MS,according to retention time,accurate relative molecular mass and standard substance comparison,these constituents were identified and speculated by Data Analysis,Metabolite Detect and other softwares,then preliminary determination of constituents absorbed into blood of rats after oral administration of extract of P. forrestii rhizomes was investigated. Result:Totally 17 constituents absorbed into blood were detected in serum,ten of them were prototype constituents and the other were metabolites.Seven of the prototypes were identified as 5-O-caffeoylquinic acid,4-O-caffeoylquinic acid,3-O-caffeoylquinic acid,3,4-di-O-caffeoylquinic acid,3,5-di-O-caffeoylquinic acid,4,5-di-O-caffeoylquinic acid and periplocin. Conclusion:These constituents absorbed into blood may be substances that act directly in vivo of P. forrestii rhizomes,and it is helpful to clarify pharmacological material basis and mechanism of this herb.