HS-SPME-GC-MS分析温郁金不同炮制品的挥发性成分

目的:对温郁金生品、醋制品和酒制品中的挥发性成分进行分析和比较,找出他们之间功效差异的物质基础,为临床合理使用温郁金不同炮制品及建立其更加完善的质量评价体系提供有力参考。方法:采用顶空固相微萃取技术(HSSPME),结合气相色谱-质谱法(GC-MS)对温郁金不同炮制品之间的挥发性成分及其相对百分含量进行对比分析。结果:从温郁金生品中初步检测出42个峰,鉴定出23个成分;从醋制品中检测出59个峰,鉴定出34个成分;从酒制品中检测出64个峰,鉴定出40个成分。与生品相比,醋制品中新增了22种成分,酒制品中新增了29种成分;温郁金不同炮制品中的挥发性成分的组成和含量均发生了变化。结论:顶空固相微萃取法操作简便、萃取速度快,不使用有机溶剂,不污染环境,操作成本小;采用顶空固相微萃取与气相色谱-质谱联合使用的方法稳定性强可靠程度高,其适用于温郁金及其不同炮制品之间挥发性成分的快速分析,可为温郁金及其不同炮制品质量评价提供一定的科学依据,并为温郁金资源的合理利用提供科学参考。

Analysis of Volatile Components of Different Processed Products of Curcuma wenyujin Radix by HS-SPME-GC-MS

Objective:To analyze and compare volatile components in raw and fried products of Curcuma wenyujin radix, and find out the material basis for the differences in efficacy between them,in order to provide powerful support and reference for the rational use of C. wenyujin radix of different processed products and establish a more perfect quality evaluation system. Method:HS-SPME combined with GC-MS was used to analyze and compare volatile components and relative percentage in different processed products of C. wenyujin radix. Result:Totally 42 peaks were detected from the raw product of C. wenyujin radix,and 23 components were identified. Totally 59 peaks were detected from vinegar-baked C. wenyujin radix,and 34 components were identified. Totally 64 peaks were detected from wine-baked C. wenyujin radix,and 40 components were identified. Compared with C. wenyujin radix,22 kinds of new ingredients were produced. Compared with C. wenyujin radix,29 kinds of new ingredients were produced in wine-baked C. wenyujin radix. The composition and content of volatile components in different processed products of C. wenyujin radix were changed. Conclusion:The headspace solid phase micro-extraction method is simple in operation and fast in extraction speed,with no use of organic solvent, no pollutant in the environment and a low operation cost. The method using headspace solid phase micro-extraction combined with gas chromatography-mass spectrometry has a high stability and reliability. It is suitable for rapid analysis of volatile components between C. wenyujin radix and its different processed products. It can provide a scientific basis for the quality evaluation of C. wenyujin radix and its different processed products,and a scientific reference for the rational use of C. wenyujin radix resources.