基于色度分析原理的青黛有效成分含量与其色度值的相关性分析

目的:研究青黛粉末外观颜色及其提取液颜色与靛蓝、靛玉红含量的相关性。方法:采用HPLC法测定27批青黛中靛蓝、靛玉红的含量,采用分光测色仪测定青黛粉末及提取液的色度值L~*,a~*,b~*。运用SPSS 20.0软件对青黛有效成分含量与其色度值进行相关性及多元线性回归分析。结果:感官评定青黛粉末外观颜色结果表明,随着粉末颜色加深,分光测色仪测定粉末和提取液的明亮度(L~*)降低、红-绿色度(a~*)升高。相关性分析表明,靛蓝含量与青黛粉末L~*呈负相关(P0.01),相关性系数为-0.520;与a~*呈正相关(P0.01),相关性系数为0.567。靛玉红含量与青黛粉末、提取液的L~*呈负相关(P0.01),相关性系数分别为-0.635,-0.580;与a~*呈正相关(P0.01),相关性系数分别为0.706,0.493。结论:青黛粉末外观颜色较深者,其靛蓝、靛玉红含量较高;青黛粉末L~*,a~*与靛蓝、靛玉红含量的相关性比提取液的相关性大;青黛粉末颜色与靛蓝、靛玉红含量具有显著相关性,为青黛快速鉴别方法提供参考依据;采用分光测色仪测定青黛色度值的方法具有稳定性、科学性和可靠性。

Correlation Between Effective Components Content and Color Values of Indigo Naturalis Basde on Chromatometry

Objective: To study the correlation between the color of Indigo Naturalis (powder and it''s extracting solution) and effective components (indigo and indirubin) content. Method: The contents of indigo and indirubin in 27 batches of Indigo Naturalis were determined by HPLC,and the chromaticity values L^*,a^* and b^* of powder and extracting solution were determined by color colorimeter. SPSS 20.0 software was used to analyze the correlation and multiple linear regression between the content of active components and the chromaticity values of Indigo Naturalis. Result: By visual observing the appearance of the powder,we find that,as the powder color deepened,the brightness values (L^*) of the powder and extract were decreased,while the red-green value (a^*) were increased. The correlation analysis showed that the contents of indigo were negatively correlated with the brightness value (L^*) of the powder(P<0.01),and the correlation coefficient was-0.520,while they were positively correlated with the red-green value (a^*)(P<0.01),and the correlation coefficient was 0.567.The contents of indirubin were negatively correlated with the brightness value (L^*) of powder and extract (P<0.01),and the correlation coefficients were-0.635 and-0.580,respectively,while they were positive correlation with the red-green value (a^*) (P<0.01),and the correlation coefficient were 0.706 and 0.493,respectively. Conclusion: The darker the powder color of Indigo Naturalis,the higher the content of indigo and indirubin. The L^* and a^* values of indigo powder were more correlated with indigo and indirubin content than with the extracting solution. The color of powder was significantly correlated with the content of indigo and indirubin,which provided reference for rapid identification of Indigo Naturalis. The method of measuring the color of Indigo Naturalis by color colorimeter is stable,scientific and reliable.