阳春砂愈伤组织的诱导

目的:通过阳春砂组织培养获得愈伤组织,建立阳春砂愈伤组织的诱导体系。方法:将阳春砂根状茎芽及阳春砂试管苗的茎段、根尖作为愈伤组织诱导的外植体材料,接种到以MS为基本培养基,分别添加不同浓度的6-苄基腺嘌呤(6-BA),ɑ-萘乙酸(NAA)及2,4-二氯苯氧乙酸(2,4-D)的培养基中(各培养基的p H约为5. 8),探讨不同外植体材料及不同培养基对阳春砂愈伤组织诱导的影响。结果:研究结果显示,阳春砂根状茎芽及试管苗的茎段、根尖3种外植体材料均能有效地诱导产生愈伤组织;其中阳春砂根状茎芽、试管苗茎段2种外植体材料诱导产生愈伤组织的最适培养基为MS+6-BA(1. 5 mg·L-1)+2,4-D(1. 0 mg·L-1)+NAA(0. 5 mg·L-1),最高诱导率分别为15%和60%;试管苗根尖诱导产生愈伤组织的最适培养基为MS+6-BA(2. 0 mg·L-1)+2,4-D(1. 0 mg·L-1)+NAA(1. 0 mg·L-1),最高诱导率76%,为阳春砂愈伤组织诱导的最佳外植体材料。结论:该研究初步建立了阳春砂的根状茎芽及试管苗的茎段、根尖3种外植体的愈伤组织诱导体系,试管苗根尖为愈伤组织诱导的最佳外植体。

Callus Induction from Amomum villosum

Objective: To set up a callus induction system for Amomum villosum by tissue culture. Method: The rhizome buds of A. villosum and stem segments,root tip segments of sterile A. villosum plantles were used as explants and cultured in MS media with different concentrations of 6-BA,NAA and 2,4-D (the pH of each medi is about 5.8). A callus induction system was established to explore the effect of different explants and different medium on callus induction for A. villosum. Result:The findings showed that the rhizome buds and sterile plantlet stems and root tip segments of three different explants can be successfully induced into calli. The most suitable medium for callus induction from rhizome buds and sterile plantlet stems was MS with 6-BA (1.5 mg·L^-1),2,4-D (1.0 mg·L^-1) and NAA (0.5 mg·L^-1) with the highest induction rates of 15%and 60%respectively. MS medium combined with 6-BA (2.0 mg·L^-1),2,4-D (1.0 mg·L^-1) and NAA (1.0 mg·L^-1) was the most suitable proposal for inducing the callus from sterile root tip segments with the highest induction rate of 76%. Conclusion:Under certain culture conditions,rhizome buds,stem or root tip segments of sterile plantlet can be effectively induced into callus. The callus induction system of A. villosum is preliminarily established, and root tip segments of sterile plantlet are the optimal explant.