Melatonin delivery by nanocapsules during in vitro bovine oocyte maturation decreased the reactive oxygen species of oocytes and embryos |
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| Affiliation: | 1. Programa de Pós-Graduação em Biotecnologia (PPGB), Grupo de Pesquisa em Oncologia Celular e Molecular, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, 96010-900 RS, Brazil;2. In Vitro Brasil S/A, Mogi Mirim, 13800-970 SP, Brazil;3. Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre 90610-000, RS, Brazil;4. Departamento de Química Orgânica, Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre 91501-970, RS, Brazil;5. Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Universidade Federal do Rio Grande, Rio Grande 96201-900, RS, Brazil;1. Federal Fluminense University (UFF), Faculty of Veterinary Medicine, Vital Brazil Filho St., 64, 24230-340 Niteroi, Rio de Janeiro, Brazil;2. Agricultural Research Company of the Rio de Janeiro State (PESAGRO RIO), São Boa Ventura Av., 770, 24120-19 Fonseca, Niteroi, Rio de Janeiro, Brazil;3. São Paulo State University (UNESP), Prof. Paulo Donato Castellane Rd., S / n, 14884-900, Jaboticabal, São Paulo, Brazil;4. Embrapa Dairy Cattle, Laboratory of Animal Reproduction, Santa Monica Experimental Field (LRA-CESM), Santa Monica Rd., 27640-000 Valença, Rio de Janeiro, Brazil;1. Laboratory of Neurogenetic and Molecular Cytogenetic, School of Science and Technology, National University of San Martin, CONICET, Buenos Aires, Argentina;2. Research Center on Surface Processes, Nanomaterials Unit, National Institute of Industrial Technology (INTI), Buenos Aires, Argentina;3. School of Pharmacy, Department of Pharmaceutical Sciences, Hampton University, Hampton, VA, USA;1. División Académica de Ciencias Agropecuarias. Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Teapa Km. 25, R/A La Huasteca 2ª Sección, ZP 86280 Villahermosa, Tabasco, Mexico;2. Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Carretera Mérida-Xmatkuil Km. 15.5, ZP, 97100 Mérida, Yucatán, Mexico;1. Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos-USP, Pirassununga-SP, Brazil;2. Departamento de Nutrição e Produção Animal, Faculdade de Medicina Veterinária e Zootecnia-USP, Pirassununga-SP, Brazil;3. WTA Watanabe Tecnologia Aplicada, Cravinhos-SP, Brazil |
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| Abstract: | In this work, a promising approach to increase the advantageous properties of melatonin through its encapsulation into lipid-core nanocapsules (LNC) was examined. Oocytes were treated during in vitro maturation with non-encapsulated melatonin (Mel), melatonin-loaded lipid-core nanocapsules (Mel-LNC), and unloaded LNC. Cytotoxicity, meiotic maturation rate, development to the blastocyst stage, reactive oxygen species (ROS) and glutathione levels, mean cell number and apoptotic cell/blastocyst, and mRNA quantification were evaluated. Both Mel and Mel-LNC enhanced in vitro embryo production, however, Mel-LNC proved to be more effective at decreasing ROS levels and the apoptotic cell number/blastocyst, increasing the cleavage and blastocyst rates, up-regulating the GPX1 and SOD2 genes, and down-regulating the CASP3 and BAX genes. Mel-LNC could penetrate into oocytes and remain inside the cells until they reach the blastocyst stage. In conclusion, when melatonin was encapsulated in LNC and applied during in vitro oocyte maturation, some quality aspects of the blastocysts were improved. |
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| Keywords: | Antioxidant Bovine oocyte Melatonin Lipid-core Nanocapsules Nanotechnology |
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