Neuroprotective effects of Caralluma tuberculata on ameliorating cognitive impairment in a d-galactose-induced mouse model |
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| Institution: | 1. Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, China;2. Department of Biotechnology, University of science and technology Bannu, K.P.K, Pakistan;3. Department of Bioinformatics and Biotechnology, Faculty of basic and applied sciences. International Islamic university Islamabad, Pakistan;4. School of basic medicine and clinical pharmacy, China Pharmaceutical University, Nanjing 210009, China;1. Department of Pharmaceutical Botany, “Iuliu Ha?ieganu” University of Medicine and Pharmacy, 23, Ghe. Marinescu Street, 400337 Cluj-Napoca, Romania;2. Institute for Life Sciences, University of Agricultural Sciences and Veterinary Medicine, Calea M?n??tur 3-5, 400372 Cluj-Napoca, Romania;3. Department of Biology, Science Faculty, Selcuk University, Konya, Turkey;4. Department of Pharmacy, University “G. d’Annunzio” of Chieti-Pescara, 66100 Chieti, Italy;5. Department of Medicinal Laboratory, Vocational School of Health Services, Selcuk University, Konya, Turkey;1. Department of Clinical Pharmacy, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu Province, 211198, People’s Republic of China;2. Department of Pharmacology, China Pharmaceutical University, Nanjing, Jiangsu Province, 211198, People’s Republic of China;3. The Research Center for Medical Genomics, China Medical University, Shenyang, Beijing, 110122, People’s Republic of China;4. Department of Pharmacy, Jiangsu Jiankang Vocational College, Nanjing, Jiangsu Province, 211198, People’s Republic of China;5. Department of Pharmacy, Jiangsu Worker Medical University, Nanjing, Jiangsu Province, 211198, People’s Republic of China;6. Department of Surgery, Nanjing Shuiximen Hospital, Nanjing, Jiangsu Province, 211198, People’s Republic of China;7. Zhongda Hospital, Affiliated with Southeast University, Nanjing, Jiangsu Province, 210017, People’s Republic of China;1. College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, People’s Republic of China;2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, People’s Republic of China;3. College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, People’s Republic of China;1. State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, PR China;2. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Bahauddin Zakariya University, Multan, 60000, Pakistan;3. School of Pharmacy, Department of Clinical Pharmacology, Nanjing Medical University, Jiangsu Province, Nanjing, 211166, PR China;4. School of Life Sciences, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210023, PR China;5. Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, PR China;6. School of Pharmacy, Nanjing Medical University, Jiangsu Province, Nanjing, 211166, PR China;7. Center for Public Health Research, Medical School of Nanjing University, Nanjing, 210023, PR China;8. Faculty of Pharmacy, University of Central Punjab, Lahore, 54570, Pakistan;1. School of Medicine, Nantong University, Nantong 226001, China;2. School of Pharmacy, Nantong University, Nantong 226001, China;3. Department of Neurochemistry, Institute for Nautical Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, China |
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| Abstract: | Cognitive deficiency and oxidative stress have been well documented in aging disorders including Alzheimer’s disease. The aim of this study was to investigate the therapeutic efficacy of Caralluma tuberculata methanolic extract (CTME) on cognitive impairment in mice induced with d-galactose. In this study we assessed the therapeutic efficacy of CTME on cognitive impairment in mice induced with d-galactose by conduction of behavioral and cognitive performance tests. In order to explore the possible role of CTME against d-galactose-induced oxidative damages, various biochemical indicators were assessed. Chronic administration of d-galactose (150 mg/kg d, s.c.) for 7 weeks significantly impaired cognitive performance (in step-through passive, active avoidance test, Hole-Board test, Novel object recognition task and Morris water maze) and oxidative defense as compared to the control group. The results revealed that CTME treatment for two weeks (100, 200 and 300 mg/kg p.o) significantly ameliorated cognitive performance and oxidative defense. All groups of CTME enhanced the learning and memory ability in step-through passive, active avoidance test, Hole-Board test Novel object recognition task and Morris water maze. Furthermore, high and middle level of CTME (300 and 200 mg/kg p.o) significantly increased Total antioxidative capacity (T-AOC), Glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) activity, neprilysin (NEP), and β-site AβPP cleaving enzyme 1 (BACE1) expression while Nitric Oxide (NO), Nitric Oxide Synthase (NOS) activity and Malondialdehyde (MDA) concentration, and the level of Aβ1-42 and presenilin 1 (PS1) were decreased. The present study showed that CTME have a significant relieving effect on learning, memory and spontaneous activities in d-galactose-induced mice model, and ameliorates cognitive impairment and biochemical dysfunction in mice. |
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| Keywords: | Alzheimer’s disease Neuroprotection Oxidative stress |
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