Induction of mice adult bone marrow mesenchymal stem cells into functional motor neuron-like cells |
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Institution: | 1. Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetic Research, Al-Mustansiriyah University, Baghdad, Iraq;2. Department of Biotechnology, Al-Nahrain University, Baghdad, Iraq;3. Neurosurgery Department, Neuroscience Hospital, Baghdad, Iraq;1. Ifremer, UMR 241, EIO, Labex Corail, Centre du Pacifique, BP 7004, 98719, Taravao, Tahiti, French Polynesia;2. SCA Regahiga Pearls, BP 48, 98755, Rikitea, Gambier, French Polynesia;3. Groupement d''Intérêt Economique Poe O Rikitea, BP 176, 98755, Rikitea, Gambier, French Polynesia;1. Dpt. de Informática, Universidad de Jaén, 23071 Jaén, Spain;2. Dpt. de Lenguajes y Sistemas Informáticos, Universidad de Granada, 28071 Jaén, Spain |
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Abstract: | The differentiation of mesenchymal stem cells (MSC) into acetylcholine secreted motor neuron-like cells, followed by elongation of the cell axon, is a promising treatment for spinal cord injury and motor neuron cell dysfunction in mammals. Differentiation is induced through a pre-induction step using Beta- mercaptoethanol (BME) followed by four days of induction with retinoic acid and sonic hedgehog. This process results in a very efficient differentiation of BM-MSCs into motor neuron-like cells. Immunocytochemistry showed that these treated cells had specific motor neural markers: microtubule associated protein-2 and acetylcholine transferase. The ability of these cells to function as motor neuron cells was assessed by measuring acetylcholine levels in a culture media during differentiation. High-performance liquid chromatography (HPLC) showed that the differentiated cells were functional. Motor neuron axon elongation was then induced by adding different concentrations of a nerve growth factor (NGF) to the differentiation media. Using a collagen matrix to mimic the natural condition of neural cells in a three-dimensional model showed that the MSCs were successfully differentiated into motor neuron-like cells. This process can efficiently differentiate MSCs into functional motor neurons that can be used for autologous nervous system therapy and especially for treating spinal cord injuries. |
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Keywords: | Mesenchymal stem cell Motor neuron Acetylcholine |
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