Neurodegenerative changes and apoptosis induced by intrauterine and extrauterine exposure of radiofrequency radiation |
| |
| Institution: | 1. Department of Biophysics, Gazi University School of Medicine and Gazi Non-Ionizing Radiation Protection Center, 06500 Ankara, Turkey;2. Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03200 Afyonkarahisar, Afyon, Turkey;3. Department of Pathology, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey;1. Unidad Funcional para el Control de la Infección Nosocomial, Hospital Universitari Arnau de Vilanova, Lleida, España;2. Servicio de Medicina Interna, Hospital Universitari Arnau de Vilanova, Lleida, España;3. Servicio de Cirugía Ortopédica y Traumatología, Hospital Universitari Arnau de Vilanova, Lleida, España;4. Atención Primaria, Área Básica de Salud Balàfia-Secà-Pardinyes, Lleida, España;1. Biostatistics Core, Nemours Biomedical Research, A I duPont Hospital for Children, Wilmington, DE 19803, United States;2. Department of Applied Economics and Statistics, University of Delaware, Newark, DE 19716, United States;1. Department of Histology and Embryology, Medical School of Ondokuz May?s University, 55139 Samsun, Turkey;2. Environmental Health Trust, 7100 N Rachel Way Unit 6 Eagles Rest, Teton Village, WY 83025, United States;1. Sinai Urban Health Institute, Sinai Health System, 1500 S. California Ave, Room K443, Chicago, IL 60608-1797, USA;2. Rosalind Franklin University, Chicago Medical School, North Chicago, IL 60064, USA;1. Boston Collaborative Drug Surveillance Program, Boston University School of Public Health, 11 Muzzey Street, Lexington, MA 02421, USA;2. Amgen Inc., Center for Observational Research, One Amgen Center Drive, Thousand Oaks, CA 91320, USA |
| |
| Abstract: | Adverse health effects of radiofrequency radiation (RFR) on the ongoing developmental stages of children from conception to childhood are scientifically anticipated subject. This study was performed to identify the effects of global system for mobile communications (GSM) modulated mobile phone like RFR in 1800 MHz frequency on oxidative DNA damage and lipid peroxidation beside the apoptotic cell formation, using histopathological and immunohistochemical methods in the brain tissue of 1-month-old male and female New Zealand White rabbits that were exposed to these fields at their mother's womb and after the birth. Oxidative DNA damage and lipid peroxidation levels were investigated by measuring the 8-hydroxy-2′-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels, respectively. Histopathological changes were observed using by hematoxylin and eosin (HE) staining. Apoptotic cells were detected in the examined organs by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining.For both male and female infants; 8-OHdG levels increased in the group exposed to RFR in both intrauterine and extrauterine periods compared to the infants that were never exposed to RFR and the ones were exposed when they reached one month of age (p < 0.05). MDA results were different for male and female rabbits. There was no difference between all female infant groups (p > 0.05), while only intrauterine exposure significantly causes MDA level increase for the male infants. HE staining revealed mild lessions in neuronal necrobiosis in brain tissues of female rabbits that had only intaruterine exposure and male rabbits had only extrauterine exposure. Gliosis were mildly positive in brain tissues of rabbits that are exposed only intrauterine period, also the group exposed both intrauterine and extrauterine periods. However, there was no apoptotic change detected by TUNEL staining in the brain tissues of all groups. |
| |
| Keywords: | Mobile phone Apoptosis DNA damage 8-OhdG Lipid peroxidation TUNEL |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
