Antibody-induced neutrophil depletion prior to the onset of pneumococcal meningitis influences long-term neurological complications in mice |
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Affiliation: | 1. Division of Critical Care Medicine, Department of Anesthesia and Critical Care, The Children''s Hospital of Philadelphia, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA;2. Department of Biomedical and Health Informatics, The Children''s Hospital of Philadelphia, Philadelphia, PA;3. Division of Infectious Diseases and the Center for Pediatric Clinical Effectiveness, The Children''s Hospital of Philadelphia, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA;1. Department of Neurology, Charité Universitätsmedizin Berlin, Germany;2. Department of Orthopedics and Traumatology, University Freiburg, Germany;3. Department of Neurology, Klinikum Klagenfurt, Austria;4. Department of Internal Medicine, Division Neurology, College of Medicine and Health Sciences, United Arab Emirates University, United Arab Emirates |
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Abstract: | During pneumococcal meningitis, clearance of bacteria by recruited neutrophils is crucial for host protection. However, these innate immune mechanisms are often insufficient and treatment with antibiotics is necessary to prevent death. Despite this antibiotic treatment, approximately half of all survivors suffer lifelong neurological problems. There is growing evidence indicating the harmful effects of neutrophils on CNS integrity. Therefore, the present study investigated the roles of neutrophils in the acute inflammatory response and the resulting long-term neuropsychological effects in murine pneumococcal meningitis. Long-term behavioural and cognitive functions in mice were measured using an automated IntelliCage system. Neutrophil depletion with antibody 1A8 as adjunctive therapy was shown to remarkably impair survival in meningitic C57BL/6J mice despite antibiotic (ceftriaxone) treatment. This was accompanied by increased bacterial load in the cerebrospinal fluid (CSF) and an increase in IL-1β, but decrease in TNF, within the CSF at 20 h after bacterial inoculation. In the longer term, the surviving neutrophil-depleted post-meningitic (PM) mice displayed reduced diurnal hypolocomotion compared to PM mice treated with an isotype antibody. However, they showed nocturnal hyperactivity, and greater learning impairment in a patrolling task that is believed to depend upon an intact hippocampus. The data thus demonstrate two important mechanisms: 1. Neutrophil extravasation into the CNS during pneumococcal meningitis influences the pro-inflammatory response and is central to control of the bacterial load, an increase in which may lead to death. 2. Neutrophil-mediated changes in the acute inflammatory response modulate the neuropsychological sequelae in mice that survive pneumococcal meningitis. |
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Keywords: | Neutrophils Pneumococcal meningitis Neurological sequelae IntelliCage Behaviour Cognition Infectious disease Central nervous system |
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