Protective effect of Tephrosia purpurea in diabetic cataract through aldose reductase inhibitory activity |
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| Affiliation: | 1. Department of Pharmacology, Institute of Pharmacy, Nirma University, Ahmedabad, Gujarat, India;2. Department of Pharmaceutical Chemistry, Institute of Pharmacy, Nirma University, Ahmedabad, Gujarat, India;3. V ClinBio Labs, Sri Ramachandra University & Hospital, Porur, Chennai, 600116, India;1. College of Life Science, Northwest Normal University, Lanzhou 730070, China;2. Bioactive Products Engineering Research Center for Gansu Distinctive Plants, Lanzhou 730070, China;3. Lanzhou Vocational Technical College, Lanzhou 730070, China;1. Chemometrics Laboratory, Faculty of Chemistry, University of Mazandaran, Babolsar 47416-95447, Iran;2. Laboratory of Bioinformatics and Drug Design, Department of Bioinformatics, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran;1. Department of Pathology, Anatomy & Cell Biology, Thomas Jefferson University, Philadelphia, PA, USA;2. Cole Eye Institute, Cleveland Clinic, Cleveland, OH, USA;3. West Virginia University Eye Institute, Morgantown, WV, USA;4. Fraternal Order of Eagles Diabetes Research Center, University of Iowa, Iowa City, IA, USA;5. Division of Endocrinology & Metabolism, Carver College of Medicine, University of Iowa, Iowa City, IA, USA;6. Department of Bio-Medical Sciences, Philadelphia College of Osteopathic Medicine, Philadelphia, PA, USA;7. Department of Cell Biology & Human Anatomy, University of California at Davis, Davis, CA, USA;8. Louis Stokes Cleveland VA Medical Center, Cleveland, OH, USA;9. Department of Ophthalmology, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH, USA;1. Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa, 62209 Cuernavaca, Morelos, Mexico;2. Laboratorio de Farmacología, Depto. Ciencias de la Salud, D.C.B.S., Universidad Autónoma Metropolitana-Iztapalapa, Apdo-Postal 55-535, CP 09340, Ciudad de México, Mexico;3. U.I.M. en Bioquímica, Hospital de Especialidades, CMNSXXI, IMSS, Ciudad de México, Mexico;4. Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, Estado de México 54090, Mexico |
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| Abstract: | PurposeTephrosia purpurea (T. purpurea) has been reported to prevent cataract formation in senile cataract model as well as proven effective in STZ induced type 1 diabetes. Aldose reductase (AR) is a key enzyme in the intracellular polyol pathway responsible for the development of diabetic cataract.ObjectiveTo investigate the effects of T. purpurea in the light of inhibition of aldose reductase enzyme in polyol pathway.MethodsWe studied the effects of alcoholic extract and flavonoid fraction of T. purpurea in streptozotocin (STZ, 45 mg/kg, i.v.)-induced type I diabetic cataract in rats. The animals were divided into five groups as control, control treated with alcoholic and flavonoid fraction, diabetic control and diabetic treated with alcoholic and flavonoid fraction. In-vitro aldose reductase inhibitory activity was also evaluated. Further, molecular docking study was performed with crystal structure of aldose reductase and its known chemical constituents of the plant.ResultsThe IC50 value of alcoholic extract for aldose reductase inhibition was found to be 209.13 μg/ml, and that of flavonoid fraction was found to be 46.73 μg/ml. Administration of STZ produced significantly abnormal levels of serum glucose, serum insulin, soluble protein and antioxidants in the lens homogenate. Treatment with alcoholic extract and flavonoid fraction of T. purpurea were able to normalize these levels. Some of the active constituents of T. purpurea showed significant interactions with aldose reductase enzyme in molecular docking studies.ConclusionsOur data suggested that both the extracts might be helpful in delaying the development of diabetic cataract due to the presence of rutin and quercetin. This beneficial effect may be due to its significant inhibition of aldose reductase enzyme and anti-oxidant activity. |
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| Keywords: | Diabetic cataract Aldose reductase Rutin |
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