Peripheral leukocyte profile in people with temporal lobe epilepsy reflects the associated proinflammatory state |
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| Institution: | 1. Neuroscience Division, Interdisciplinary Laboratory of Medical Investigation, School of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil;2. Epilepsy Treatment Advanced Centre (NATE), Felício Rocho Hospital, Belo Horizonte, MG, Brazil;3. Medicine School, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, MG, Brazil;4. Laboratório de Imunologia do Envelhecimento, Instituto de Pesquisas Biomédicas, PUCRS, Porto Alegre, RS, Brazil;5. Stichting Epilepsie Instellingen Nederland (SEIN), Heemstede, The Netherlands;6. NIHR University College London Hospitals Biomedical Research Centre, UCL Institute of Neurology, Queen Square, London WC1N 3BG, Epilepsy Society, Chalfont St Peter, SL9 0RJ, UK;7. Neurology Department, UNICAMP, Campinas, SP, Brazil;1. Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA;2. Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN 46202, USA;3. Collaborative Core for Cancer Bioinformatics, Indiana University Simon Cancer Center, Indianapolis, IN 46202, USA;4. Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, IN 46202, USA;5. Purdue Institute for Inflammation, Immunology, & Infectious Disease, Purdue University, West Lafayette, IN 47907, USA;6. Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA;1. Harran University School of Medicine, Department of Neurology, ?anl?urfa, Turkey;2. Harran University School of Medicine, Department of Biochemistry and Clinical Biochemistry, ?anl?urfa, Turkey;1. Department of Physics, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia;2. Department of Physics, Faculty of Science, Al-Azhar University, Assiut, Egypt;3. Department of Pharmaceutics, College of Pharmacy, King Saud University, Saudi Arabia;4. Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Al-Azhar University, Assiut, Egypt;1. Department of Neurology, Zhongshan Hospital, Fudan University, Shanghai, China;2. Institute of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China;3. Department of Radiology, Zhongshan Hospital, Fudan University, Shanghai, 200031, China;4. Institute of Science and Technology for Brain-Inspired Intelligence, Fudan University, Shanghai, 200433, China;5. The State Key Laboratory of Medical Neurobiology, The Institutes of Brain Science and the Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, China;1. Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China;2. Department of Neurosurgery, Chengdu Military General Hospital, Sichuan 610083, China;3. Department of Neurosurgery, Nanchong Central Hospital, Sichuan 637900, China |
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| Abstract: | IntroductionMarkers of low-grade peripheral inflammation have been reported amongst people with epilepsy. The mechanisms underlying this phenomenon are unknown. We attempted to characterize peripheral immune cells and their activation status in people with temporal lobe epilepsy (TLE) and healthy controls.Methods and resultsTwenty people with TLE and 19 controls were recruited, and peripheral blood lymphocyte and monocyte subsets evaluated ex vivo by multi-color flow cytometry. People with TLE had higher expression of HLA-DR, CD69, CTLA-4, CD25, IL-23R, IFN-γ, TNF and IL-17 in CD4+ lymphocytes than controls. Granzyme A, CTLA-4, IL-23R and IL-17 expression was also elevated in CD8+ T cells from people with TLE. Frequency of HLA-DR in CD19+ B cells and regulatory T cells CD4+CD25+Foxp3+ producing IL-10 was higher in TLE when compared with controls. A negative correlation between CD4+ expressing co-stimulatory molecules (CD69, CD25 and CTLA-4) with age at onset of seizures was found. The frequency of CD4+CD25+Foxp3+ cells was also positively correlated with age at onset of seizures.ConclusionImmune cells of people with TLE show an activation profile, mainly in effector T cells, in line with the low-grade peripheral inflammation. |
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| Keywords: | Human temporal lobe epilepsy Immunophenotyping Lymphocytes Cell activation Cytokines |
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