Biological activities of skin and parotoid gland secretions of bufonid toads (Bufo bufo,Bufo verrucosissimus and Bufotes variabilis) from Turkey |
| |
| Institution: | 1. Ege University, Faculty of Engineering, Department of Bioengineering, 35100 Bornova, Izmir, Turkey;2. Ege University, Faculty of Science, Department of Biology, Zoology Section, 35100 Bornova, Izmir, Turkey;3. Ege University, Faculty of Science, Department of Biology, Basic and Industrial Microbiology Section, 35100 Bornova, Izmir, Turkey;1. Recep Tayyip Erdo?an University, Faculty of Arts and Sciences, Department of Biology, Rize, Turkey;2. Recep Tayyip Erdo?an University, Faculty of Fisheries, Rize, Turkey;1. Guizhou Provincial College-based Key Lab for Tumor Prevention and Treatment with Distinctive Medicines, Zunyi Medical University, Zunyi, Guizhou, 563000, China;2. Research Center for Medicine & Biology, Zunyi Medical University, Zunyi, Guizhou, 563000, China;1. Key Laboratory of Xin''an Medicine, Ministry of Education, Anhui Province Key Laboratory of R&D of Chinese Medicine, Anhui University of Chinese Medicine, Hefei, 230038, China;2. Scientific Research & Experiment Center, Anhui University of Chinese Medicine, Hefei, 230038, China;3. Anhui China Resources Jin Chan Pharmaceutical Co., Ltd., Huaibei, 235000, China;4. Basic Medical College, Anhui Medical University, Hefei, 230032, China;1. Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, 64.049-550, Teresina, Piauí, Brazil;2. Department of Biological Sciences, Campus Senador Helvídio Nunes de Barros, Federal University of Piauí, 64607-670, Picos, Piauí, Brazil;3. Department of Chemistry, Federal University of Piauí, 64.049-550, Teresina, Piauí, Brazil;4. Department of Biophysics and Physiology, Laboratory of Experimental Cancerology, Federal University of Piauí, 64.049-550, Teresina, Piauí, Brazil |
| |
| Abstract: | Toad glandular secretions and skin extractions contain numerous natural agents which may provide unique resources for novel drug development. Especially the skin-parotoid gland secretions of toads from genus Bufo contain as many as 86 different types of active compounds, each with the potential of becoming a potent drug. In the present study, crude skin-parotoid gland secretions from Bufo bufo, Bufo verrucosissimus and Bufotes variabilis from Turkey were screened against various cancer cells together with normal cells using MTT assay. Furthermore, the antimicrobial properties of skin secretions were tested on selected bacterial and fungal species for assessing the possible medical applications. Antimicrobial activity of skin secretions was studied by determining minimal inhibitory concentration (MIC) in broth dilution method. Hemolytic activity of each skin-secretion was also estimated for evaluating pharmaceutical potential. Both skin-parotoid gland secretions showed high cytotoxic effect on all cancerous and non-cancerous cell lines with IC50 values varying between <0.1 μg/ml and 6.02 μg/ml. MIC results of antimicrobial activity tests were found to be between 3.9 μg/ml and 250 μg/ml. No hemolytic activities on rabbit red blood cells at concentrations between 0.5 μg/ml and 50 μg/ml were observed. In conclusion, skin-parotoid secretions of bufonid toads might be remarkable candidates for anti-cancer and antimicrobial agents without hemolytic activities. |
| |
| Keywords: | Amphibian skin secretion Cytotoxicity Antimicrobial activity Hemolytic activity |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
