| GPC3/MXR7基因在肾细胞癌及癌旁肾组织中的表达 |
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| 引用本文： | 史立新,陈文政,张磊,丛冰,张旭,那彦群.GPC3/MXR7基因在肾细胞癌及癌旁肾组织中的表达[J].中华实验外科杂志,2008,25(10). |
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| 作者姓名： | 史立新 陈文政 张磊 丛冰 张旭 那彦群 |
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| 作者单位： | 1. 解放军总医院泌尿外科,北京,100853
2. 北京大学第一医院泌尿外科 |
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| 摘 要： | 目的 探讨肾癌、癌旁肾组织中多种相关基因的表达水平及差异,以及与临床分期的关系.方法 应用实时荧光定量PCR方法 检测GPC3/MXR7基因在肾细胞癌组织及癌旁肾组织中的表达,并比较不同临床阶段其表达的差异.结果 GPC3/MXR7在肾癌组织中平均表达(GPC3/MXR7和18 S的比值)是(0.07±0.14)×10-3,而癌旁肾组织中是(0.16±0.14)×10-3,差异有统计学意义(P＜0.05),表明GPC3/MXR7基因在肾癌组织中平均表达均明显低于在癌旁肾组织中的平均表达.GPC3/MXR7在癌旁与肾癌的表达比值在临床分期(I+Ⅱ)期中平均为32.05±48.87,而在(Ⅲ+Ⅳ)期中为22.95±49.58,差异无统计学意义(P＞0.05),表明GPC3/MXR7基因在癌旁与肾癌组织中的表达降低或增高均与临床分期无关.结论 肾癌的发生、发展可能与GPC3/MXR7基因有关,是多种基因共同作用的结果 .
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| 关 键 词： | 肾细胞癌 实时荧光定量聚合酶链反应 基因表达 |
Expression of GPC3/MXR7 gene in renal cell carcinoma and adjacent renal tissue |
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| Abstract: | Objective To investigate the expression of GPC3/MXR7 genes in renal carcinoma tissues and carcinoma-adjacent renal tissues and the relationship with clinical staging. Methods Realtime fluorescence quantitative PCR method was used to detect the expression level of GPC3/MXR7 in renal carcinoma tissues and carcinoma-adjacent renal tissues and to compare the expression difference in different clinical stages. Results The expression level of GPC3/MXR7 gene in renal carcinoma tissues ( 0.07 × 10-3 0. 14 × 10 -3 ) was obviously lower than in carcinoma-adjacent renal tissues (0. 16 × 10-3 0.14 × 10 -3 ) (p ＜ 0.05 ). The GPC3/MXR7 ratio of paracancer to cancer in Ⅰ + Ⅱ stages was 32.05 ± 48.87 and that in Ⅲ + Ⅳ stages was 22.95 ± 49.58 with the difference being not significant ( P ＞ 0.05 ). Condusion GPC3/MXR7 might contribute to the occurrence and progression of renal caricinoma. |
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| Keywords: | Renal cell carcinoma Real-time fluorescence quantitative PCR Gene expression |
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