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| PCR检定eNOS基因VNTR多态性 | |
| 引用本文： | 路萍,吕星,邢瑞云,孙琪云,钱瑞华,邱泽武,张少华,洪权,郑晓飞.PCR检定eNOS基因VNTR多态性[J].军事医学科学院院刊,2001,25(3):171-173. |
| 作者姓名： | 路萍 吕星 邢瑞云 孙琪云 钱瑞华 邱泽武 张少华 洪权 郑晓飞 |
| 作者单位： | 1. 北京军医学院生物化学教研室 2. 军事医学科学院放射医学研究所 3. 军事医学科学院附属医院 |
| 基金项目： | 国家“973”重点基础研究发展规划项目(G2 0 0 0 0 5 70 0 1) |
| 摘 要： | 目的：建立eNOS基因基因VNTR多态性的PCR分析方法。方法：按eNOS基因VNTR位点侧翼序列设计引物，自健康献血者基因组DNA扩增VNTR片段，琼脂糖凝电泳分析扩增片段的长度和数目，鉴定VNTR基因型。结果与结论：在208名健康献血者中鉴定出6／5杂合、5／5纯合、5／4杂合和4／4纯合4种VNTR基因型，等位基因的分布频率与日本人相似，但与美国黑人存在显著差异。 |
| 关 键 词： | 一氧化氮合酶 基因多态性 数目可变串联重复序列 聚合酶链反应 eNOS基因 |
| 文章编号： | 1000-5501(2001)03-0171-03 |
| 修稿时间： | 2001年4月17日 |
Detection of the VNTR polymorphisms in eNOS gene by PCR method |
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| LU Ping,L? Xing,XING RuiYun,SUN QiYun,QIAN Rui Hua,QIU ZeWu,ZHANG ShaoHua,HONG Quan,ZHENG XiaoFei.Detection of the VNTR polymorphisms in eNOS gene by PCR method[J].Bulletin of the Academy of Military Medical Sciences,2001,25(3):171-173. | |
| Authors: | LU Ping L? Xing XING RuiYun SUN QiYun QIAN Rui Hua QIU ZeWu ZHANG ShaoHua HONG Quan ZHENG XiaoFei |
| Institution: | LU Ping2,L? Xing1,XING RuiYun1,SUN QiYun3,QIAN Rui Hua3,QIU ZeWu3,ZHANG ShaoHua3,HONG Quan1,ZHENG XiaoFei1 |
| Abstract: | ObjectiveTo establish the PCR method for analy si s and detection of the variable number of tandem repeats(VNTR)polymorphism in e~{!<~}WT~{!=~}NOS gene. MethodsWith the use of primers desig ned according to the flanking sequences of VNTR locus, VNTR fragments were amplified fro m genomic DNA of healthy subjects. For VNTR genotyping, the length and number of repeats were analyzed through agarose gel electrophoresis. Results and conclusionsFour genot ypes of VNTR, namely 6/5repeats heterozygous, 5/5repeats homozygous, 5/4repeats heterozygous and 4/4 repea ts homozygous, were identified from a total of 208 healthy subjects. The overall distribution of all ele frequencies was similar to that of Japanese, while it differed significantly from that of African Americans. |
| Keywords: | nitric oxide synthase gene polymorphism variabl e number of tandem repeats |
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