干燥综合征患者唇腺淋巴细胞分类观察

用卵白素—生物素过氧化酶复合物技术对15例原发性干燥综合征(SS)、7例继发性干燥综合征和4例正常人唇腺中浸润的淋巴细胞进行了分类观察。在SS患者唇腺浸润的淋巴细胞中,以T细胞(CD3 )为主,而住T细胞中又以辅助诱导性T细胞(CD4 )占多数,CD4/CD8的比值明显高于对照组,并且与疾病的严重程度有关。唇腺中的B细胞明显高于对照组,B细胞的升高与血清γ-球蛋白和唾液腺分泌型IgA升高有关。大多数淋巴细胞都有HLA-DR的表达,一些患者腺上皮细胞也有HLA-DR的表达。由于T辅助细胞数增多或功能增强,T抑制细胞数减少或功能不足,导致B淋巴细胞功能亢进,产生大量的自身抗体。大量淋巴细胞侵袭唾液腺使其组织结构被破坏,腺体功能减弱或丧失。由于出现免疫失调,机体的其它组织和机能也遭到破坏。

Lymphocyte Subsets in Labial Salivary Gland of Sjogren's Syndrome

Using the immunohistochemical avidin-biotin-peroxidase complex (ABC) method, Lymphocyte subsets in the salivary glands of 15 patients with primary Sjogren's syndrome, 7 patients with secondary Sjogren's syndrome and 4 nor- mal controls were identified by monoclonal antibodies. The infiltrating lymphocytes were mainly T lymphocytes, the majority of which were T helper cells (Leu-3a) in SS groups. The Leu-3a/Leu-2a ratio was increased. These changes were related to the degrees of the disease. The lymphocytes of patients expressed the HLA-DR antigen (Ia). Thus genetic predisposition plays an important role in the disease. A proposed mechanism is that the deficiency of suppressor lymphocytes and the overactivity of helper lymphocytes would result in B cells becoming hyperactive. Multiple autoantibodies produced by the B cells would contribute to tissue damage, and the tissue fragments would in turn further stimulate antibody production.