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| 阿加曲班对大鼠脑出血后血肿周围基质金属蛋白酶2表达的影响 | |
| 引用本文： | 崔桂云,王庆广,赵辉,陆军,葛巍,张作慧,沈霞. 阿加曲班对大鼠脑出血后血肿周围基质金属蛋白酶2表达的影响[J]. 中国脑血管病杂志, 2008, 5(12) |
| 作者姓名： | 崔桂云 王庆广 赵辉 陆军 葛巍 张作慧 沈霞 |
| 作者单位： | 1. 徐州医学院附属医院神经内科,江苏省,221002 2. 江阴市人民医院神经内科 3. 徐州市中心医院神经内科 |
| 基金项目： | 江苏省高校自然科学研究指导性计划研究项目 |
| 摘 要： | 目的探讨阿加曲班对大鼠脑出血后血肿周围组织基质金属蛋白酶2(MMP-2)表达的影响。方法采用自体血注入法建立大鼠脑出血模型,于脑出血后3h,颅内原位注入阿加曲班(0.5μg/ml)。分别用Westernblot和免疫组化方法检测血肿周围组织MMP-2的表达,伊文思蓝(EB)测血脑屏障(BBB)通透性,干湿重法测脑组织含水量,并与假手术组相比较。结果在脑出血后24h,同侧基底核MMP-2的表达至高峰,脑出血组、等渗盐水组和阿加曲班干预组的MMP-2阳性细胞数分别为21.8±0.9、3.2±0.4和14.9±1.0;EB含量分别为(20.8±0.9)、(4.6±0.7)和(15.4±0.9)ng/mg;脑组织含水量分别为(84.3±1.1)、(78.7±1.5)和(81.2±1.6)%。脑出血组与等渗盐水组及阿加曲班干预组同侧基底核的MMP-2表达、EB含量和脑组织含水量比较,差异均有统计学意义(P<0.01,P<0.05)。结论凝血酶可能通过促进MMP-2的表达,诱导脑出血后脑水肿的形成,阿加曲班能明显减轻大鼠脑出血后血-脑屏障损伤和血肿周围脑组织的水肿。 |
| 关 键 词： | 脑出血 阿加曲班 基质金属蛋白酶2 凝血酶 大鼠 |
Effect of argatroban on the expression of matrix metalloproteinase-2 in perihematomal tissue after intracerebral hemorrhage in rats |
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| CUI Gui-yun,WANG Qing-guang,ZHAO Hui,LU Jun,GE Wei,ZHANG Zuo-hui,SHEN Xia. Effect of argatroban on the expression of matrix metalloproteinase-2 in perihematomal tissue after intracerebral hemorrhage in rats[J]. Chinese Journal of Cerebrovascular Diseases, 2008, 5(12) | |
| Authors: | CUI Gui-yun WANG Qing-guang ZHAO Hui LU Jun GE Wei ZHANG Zuo-hui SHEN Xia |
| Affiliation: | CUI Gui-yun; WANG Qing-guang; ZHAO Hui; LU Jun; GE Wei; ZHANG Zuo-hui; SHEN Xia.Department of Neurology; the Affiliated Hospital of Xuzhou Medical College; Xuzhou 221002; China; |
| Abstract: | Objective To investigate the effect of argatroban on the expression of matrix metalloproteinase-2(MMP-2)in perihematomal tissue after intracerebral hemorrhage(ICH)in rats.Methods A rat model of ICH was induced by autologous blood injection.In situ injection of argatroban(0.5 μg/mL)into hematoma was performed 3 hours after ICH.Then the expression of MMP-2 in perihematomal tissue was detected by Western blot and immunohistochemistry,respectively;the permeability of blood-brain barrier(BBB)was assessed by Evan... |
| Keywords: | Cerebral hemorrhage Argatroban Matrix metalloproteinase2 Thrombin Rats |
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