microRNA-181c在重症肌无力患者外周血单个核细胞中的表达及其意义

目的探讨microRNA-181c(miR-181c)在重症肌无力(myasthenia gravis,MG)患者中的表达水平及其临床意义。方法收集2015年6月-2017年6月收治的22例MG患者和20例健康人的外周静脉血样,提取外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中总RNA,采用实时定量PCR(quantitative real-time PCR,qRT-PCR)技术检测PBMC中miR-181c的相对表达水平,并分析miR-181c表达水平与患者的MG定量评分体系(quantitative myasthenia gravis score,QMG)的相关性。结果 MG患者PBMC中miR-181c的相对表达水平(0.303±0.150)较对照组(1.000±0.262)显著降低(P0.01),其中全身型患者(0.182±0.056)低于眼肌型患者(0.404±0.126)(P0.05),同时miR-181c表达水平与QMG呈负相关(R2=-0.359,P0.05)。结论 MG患者PBMC中miR-181c表达水平的降低可能与病情严重程度有关。

Expression of microRNA-181 c in peripheral blood mononuclear cells from patients with myasthenia gravis and its significance

Objective To evaluate the expression and clinical significance of miR-181c in patients with myasthenia gra-vis.Methods Plasma blood samples from 22 patients with MG and 20 healthy subjects were collected from June 2015 to June 2017,and the total RNA was extracted from peripheral blood mononuclear cells .To detect the relative expression of miR-181c in PBMC by Real-time quantitative polymerase chain reaction and to analyze the correlation between the expression level of miR-181c and the quantitative myasthenia gravis score (QMG) in MG patients.Results The relative expression level of miR-181c in PBMC of MG patients (0.303 ±0.150) was significantly lower than that of control group (1.000 ±0.262) (P<0.01),and the level of miR-181c in generalized patients (0.182 ±0.056) was lower than that in ocular patients (0.404 ±0.126) (P<0.05), meanwhile the expression level of miR-181c was negatively correlated with QMG (R2=-0.359,P<0.05).Conclusion The decrease of miR-181c expression in PBMC of MG patients may be related to the severity of the disease .