脑源性神经生长因子对mir124表达及兴奋性电流的影响

目的探讨BDNF对海马miRNA-124表达及齿状回颗粒细胞兴奋性突触后电流(s EPSCs)的影响,以明确BDNF对颞叶内侧癫痫(MTLE)发病机制的影响。方法选取哈尔滨医科大学附属第一临床医院神经外科2008年4月-2010年10月手术治疗的MTLE患者12例。RT-pcr技术检测BDNF孵育后MTLE患者海马组织mir-124表达、膜片钳技术检测BDNF对海马颗粒细胞s EPSCs的影响。结果 (1)BDNF降低了颞叶癫痫海马miRNA-124的表达(P0.01);(2)BDNF增加了颗粒细胞sEPSCs的频率和幅度(P0.01)。结论 BDNF降低了颞叶癫痫海马mir-124的表达,提高颗粒细胞sEPSCs的频率和幅度,从而可能对人类MTLE的发病起到促进作用。

The effect of BDNF on levels of miRNA-124 and sEPSCs

Objective To study the effect of brain derived neurotrophic factor (BDNF) in the pathogenesis from patients with MTLE.Methods The study was performed between April 2008 and October 2010.Surgically removed specimens were collected from the patients with MTLE.All patients gave written informed consent for research use of the biopsy materials.Surgically resected hippocampal were collected and immediately immersed in oxygenated ice-cold SACSF.The expression of Mir-124 was detected by RT-PCR and sEPSCs by patch-clamp.Results Expression of mir-124 was significantly decreased in Dentate Gyrus of MTLE compared with that of controls (P<0.01).BDNF increases frequency and amplitude of sEPSC (P<0.01).Conclusions The results indicate that BDNF significantly decreased the expression of mir-124 as in Dentate Gyrus from patients with MTLE.Our electrophysiological findings indicate that BDNF increase excitability on dentate granule cells.In conclusion,our work supports a role of BDNF in pathophisyology of MTLE and raises the possibility that interference with BDNF action may be a therapeutic method for patients of MTLE.