| 大鼠心肌成纤维细胞合成金属硫蛋白 |
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| 引用本文： | 高霖,王冬艳,李扬,石彦荣,卜定方,庞永正,唐朝枢.大鼠心肌成纤维细胞合成金属硫蛋白[J].北京大学学报(医学版),2003,35(1):41-44. |
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| 作者姓名： | 高霖 王冬艳 李扬 石彦荣 卜定方 庞永正 唐朝枢 |
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| 作者单位： | 1. 北京大学第一医院心血管病研究所,北京,100034
2. 北京大学医学部临床医学系 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);G200056905; |
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| 摘 要： | 目的 :探讨脂多糖 (lipopolysaccharide ,LPS)、碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor ,bFGF)、氧化型低密度脂蛋白 (oxidizedlow densitylipoproteins ,oxLDL)及重金属物质ZnCl2 等刺激因素对大鼠心肌成纤维细胞金属硫蛋白 (metallothionein ,MT)合成的影响。方法 :用10 9Cd Hb饱和法测定细胞MT含量 ,用RT PCR法测定细胞MTmRNA含量。结果 :本实验中各种处理因素均可刺激心肌成纤维细胞MT含量的增加。 1和5mg·L-1LPS处理组成纤维细胞MT含量与对照组相比分别增加 94 %和 130 % (P <0 .0 1)。 0 .2和 1.0mg·L-1bFGF处理组分别增加 4 1%和 5 9% (P <0 .0 1)。oxLDL亦可以促进心肌成纤维细胞MT的合成 ,2和 10mg·L-1oxLDL组MT含量较对照组分别增加 2 3% (P <0 .0 5 )和 4 1% (P <0 .0 1)。 1,2 ,5mg·L-1ZnCl2 呈浓度依赖性刺激心肌成纤维细胞MT含量的增加 ,与对照组相比分别增加 6 6 %、99%和 14 9% (P <0 .0 1) ,2～ 8mg·L-1ZnCl2 诱导MT1mRNA含量增加 6 9%～ 12 9% (P <0 .0 1) ,MT2mRNA含量增加 31%～ 6 0 % (P <0 .0 1)。但 10mg·L-1ZnCl2 不能进一步增加细胞MT含量 ,仅较对照组增加 91% ,可能与高浓度ZnCl2 致细胞损伤 ,细胞存活率降低有关。结论 :LPS、bFGF、oxLDL、ZnCl2 等多
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| 关 键 词： | 心肌 成纤维细胞 金属硫蛋白 生物合成 |
| 文章编号： | 1671-167X(2003)01-0041-04 |
The synthesis of MT in rat myocardial fibroblasts |
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| Lin Gao,Dongyan Wang,Yang Li,Yanrong Shi,Dingfang Bu,Yongzheng Pang,Chaoshu Tang.The synthesis of MT in rat myocardial fibroblasts[J].Journal of Peking University:Health Sciences,2003,35(1):41-44. |
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| Authors: | Lin Gao Dongyan Wang Yang Li Yanrong Shi Dingfang Bu Yongzheng Pang Chaoshu Tang |
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| Institution: | Institute of Cardiovascular Research, Peking University First Hospital, Beijing 100034, China. |
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| Abstract: | OBJECTIVE: To study the influence on the synthesis of metallothionein (MT) induced by lipopolysaccharide (LPS), basic fibroblast growth factor (bFGF), oxidized low-density lipoproteins (oxLDL) and ZnCl2 in rat myocardial fibroblasts. METHODS: The content of MT was measured by 109Cd-Hb saturation method and RT-PCR was used to determine the expression of MT mRNA. RESULTS: All of the stimulus in this experiment increased the synthesis of MT in myocardial fibroblasts. Compared with control, 1 and 5 mg.L-1 LPS increased the content of MT by 94% and 130% (P < 0.01), 0.2 and 1.0 mg.L-1 bFGF by 41% and 59% (P < 0.01), 2 and 10 mg.L-1 oxLDL by 23% (P < 0.05) and 41% (P < 0.01) respectively. 1,2 and 5 mg.L-1 ZnCl2 increased the content of MT by 66%, 99% and 149% (P < 0.01) in a concentration-dependent manner. 2-8 mg.L-1 ZnCl2 increased the content of MT1 mRNA by 69%-129%, MT2 mRNA by 31%-60% (P < 0.01). But 10 mg.L-1 ZnCl2 didn't stimulate the synthesis of MT more significantly, probably because ZnCl2 of high concentration damaged myocardial fibroblasts and decreased the survival rate of the cells. CONCLUSION: Many stimulus such as LPS, bFGF, oxLDL and ZnCl2 can induce the synthesis of MT, and ZnCl2 can increase the expression of MT mRNA in rat myocardial fibroblasts. |
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| Keywords: | Myocardium Fibroblasts Metallothionein/biosyn |
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