单胺类递质在大鼠癫痫发作心脏损伤中的作用

目的:观察癫痫发作前、发作时及发作后血浆去甲肾上腺素(NE)及血清心肌肌钙蛋白I(cTnI)含量以及心脏病理变化。探讨癫痫发作致心脏损害的发生、发展机制。方法:健康Wistar大鼠40只随机分成癫痫组(EP)和对照组(Control)。癫痫由青霉素诱导发作,动态监测大鼠癫痫发作时和发作后不同时间点血浆NE含量及血清cTnI水平的变化。NE的测定采用高效液相-电化学法,cTnI的测定采用光化学发光法。结果:大鼠癫痫发作时血浆NE明显升高,NE升高持续约1h以上。癫痫发作后1h血清cTnI水平开始升高,12h达高峰,随后逐渐下降。心脏病理检查证实癫痫发作可引起心脏损害。结论:癫痫发作时NE含量升高在癫痫发作致心脏损害过程中起重要作用。

The effects of monoamine nervous transmitter on myocardial injury during epileptic seizures in rats

Objective:To observe the changes of plasma noradrenaline(NE)and serum cardiac tropin I(cTnI)during epileptic seizure and after that in different time,and discuss the mechanism of cardiac injury result from epileptic seizure.Methods:40 adult healthy Wistar rats were randomly divided into epilepsy group and control group,and epileptic seizures were induced by penicillin.NE and cTnI were determined by high-performance-1iquid chromatography and automated chemiluminescence method.We observed the changes of plasm NE and serum cTnI during and after seizure at 1h,6h,12h,24h and 48h,respectively.Results:NE activity in plasma obviously increased during epileptic seizure,which lasted about 1 hour.Serum cTnI elevated at 1h after seizure,and reached the peak at 12h,then gradually decreased.Cardiac injury during epileptic seizure was proved by pathologic examination.Conclusion:The enhanced activity of peripheral NE may play an important role in cardiac injury result from epileptic seizure.