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微柱凝胶法抗体筛查的结果分析
引用本文:沈轶骊,王雪明,虞秀兰,彭群新,李勇,刘冬梅,周志英.微柱凝胶法抗体筛查的结果分析[J].中国血液流变学杂志,2009,19(3):471-472.
作者姓名:沈轶骊  王雪明  虞秀兰  彭群新  李勇  刘冬梅  周志英
作者单位:苏州大学附属第一医院输血科,江苏苏州,215006
摘    要:目的围手术期和内科输血的患者进行不规则抗体筛查,以减少或杜绝溶血性输血反应的发生。方法用微柱凝胶Coombs卡对35,210例标本进行不规则抗体筛选,阳性标本用盐水、酶、抗人球蛋白、结合谱细胞和各种血型定型试剂确定抗体特异性。结果微柱凝胶Coombs卡筛选检出不规则抗体阳性标本83例,阳性率0.236%。结论微柱凝胶Coombs卡抗体筛选能有效降低和避免溶血性输血反应的发生.保证输血的安余性。

关 键 词:不规则抗体  微柱凝胶Coombs试验  溶血性输血反应

The Results of Antibody Screening with Microcolum Gel Coombing
Institution:SHEN Yi-li, WANG Xue-ming, YU Xiu-lan, et al (Dept of Blood Transfusion,First Affiliated Hospital,Soochow University,Suzhou,Jiangsu,215006)
Abstract:Objective To screen the irregular antibody to induce or stop hemolytic blood transfusion reaction for the perioperative period and the transfusion of internal medicine.Method Screening the 35,210 samples' irregular antibody with microcolum gel coombs card,confirming antibody's specifity with brine,enzyme,antihuman globulin, combining cells and each blood typing reagents.Result Microcolum gel coombs card can screen 83 positive samples in irregular antibody,the positive is 0.236%.Conclusion Microcolum gel coombs card for antibody screening can efficiently bring down and stop the hemolytic blood transfusion reaction, which can ensure the salty of blood transfusion.
Keywords:irregular antibody  microcolum gel coombs test  hemolytic blood transfusion
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