小儿急性淋巴细胞白血病髓系抗原和CD_(34)表达的分析

为分析小儿急性淋巴细胞白血病 (ALL)的髓系抗原、CD34 表达特点 ,采用间接免疫荧光法分析 6 2例初治的ALL患者的免疫表型。结果①儿童ALL髓系抗原阳性率达 2 0 2 % ,其中CD13阳性最常见 (占 1 2 % )T系ALL和B系ALL髓系抗原表达差异无显著性。ALL L2 中的阳性率高于L1(P <0 0 5)。②CD34 表达阳性率为33 3% ,B ALL阳性率高于T ALL(P <0 0 1 ) ,B ALL中前B细胞型明显高于普通型、B细胞各亚型。③CD34 表达与外周血幼稚细胞有关 (P <0 0 1 )。结果表明儿童ALL髓质抗原表达和FAB分型有关 ;CD34 表达与外周血原始细胞和脑膜白血病的发生有关。与ALL亚型、细胞分化程度有关。

Study on the expression of myeloid markers and CD34 antigen in children acute lymphoblastic leukemia

To analyse the expression of myeloid markers and CD 34 antigen on lymphoblasts in childhood acute lymphoblastic luekemias (ALL) and its relationship with prognosis. Method: lmmunoph enotypes in 62 cases of ALL were determined byindirect immunoflurosescence method. Results: (1) The incidence of myeloid antigen expression in childhood ALL was 20.2% and the commonest one was CD 34 (12%). There was a higher incidence of myeloid antigens expresion in ALL-L 2 than in ALL-L 1 (p<0.05) (2) CD 34 was expressed of in lymphoblasts from 14 of 42 patients (33.3%), incidence CD 34 expression in B-ALL was higher than that in T-ALL (p<0.01), pre-B-ALL was higher than common and B-ALL in CD 34 (3)The expression of CD 34 was related to brain luekemia. (p<0.01). (4)The expression of CD 34 was related to the average of original cell counts (P<0.01). Conclusion: Expression of myeloid antigens was related to FAB subtype in childhood ALL. The expression of CD 34 was related to the average of original cell counts with brain leukemia. Pre-B-ALL was higher than common and B-ALL in CD 34 .