Exercise-induced changes in the expression of surface adhesion molecules on circulating granulocytes and lymphocytes subpopulations |
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Authors: | Y. Kurokawa S. Shinkai J. Torii S. Hino P. N. Shek |
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Affiliation: | (1) Departments of Hygiene and Public Health, Ehime University School of Medicine, Shigenobu-cho, Onsen-gun, 791-02 Ehime, Japan;(2) Department of Medical Laboratory Technology, Ehime College of Health Science, Tobe-cho, Iyo-gun, 791-21 Ehime, Japan;(3) Department of Nutritiopn, Yamaguchi Women's College, Sakurabatake, Yamaguchi-shi, 753 Yamaguchi, Japan;(4) Operation Medicine Division, Defence and Civil Institute of Environmental Medicine, M3M 3B9 North York, Ontario, Canada;(5) Department of Clinical Biochemistry, Faculty of Medicine, University of Toronto, M5S 1A8 Toronto, Ontario, Canada |
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Abstract: | This study examined the relationship between exercise-induced changes in the concentration of circulating immunocompetent cells and their surface expression of adhesion molecules: L-selectin (CD62L) and three 2-integrins [LFA-1(CD11a/CD18), Mac-1 (CD11b/CD18), and p150/95(CD11c/CD18)]. Eight young male volunteers exercised on a cycle ergometer for 60 min at 60% maximal oxygen uptake. Peripheral blood samples, collected every 30 min throughout exercise and during the 2-h recovery period, were used for flow-cytometric analysis. The experimental results were compared with control data obtained ever 60 min at corresponding times of the nonexercise day. The exercise regimen induced a granulocytosis and a lymphocytosis, mainly due to an elevation of CD8+ and CD16+ cells. During recovery, a further granulocytosis occurred but accompanied by a lymphopenia. The increased CD8+ cell-count during exercise was characterized by a selective mobilization of the CD62L– and CD11ahigh cells, i.e.primed CD8+ cells. A postexercise suppression of CD4+ cell-count was derived only from CD62L+ cells. The CD11b+ and CD11c+ lymphocytes also increased during exercise, largely attributable to an increase in CD16+ cells which co-expressed CD11b and CD11c molecules. The CD62L surface density of granulocytes increased significantly during recovery. This resulted from a selective influx of CD62Lhigh granulocytes into the circulation. There were no significant changes in per-cell density of the three 2-integrins on granulocytes and lymphocytes throughout the experimental period. These results suggest that the cell-surface expression of CD62L (and CD I la) molecules is associated with the differential mobilization of CD8+ cells during exercise, the postexercise suppression of CD4– cell-counts and the granulocytosis following exercise. |
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Keywords: | Physical exercise Leukocyte Adhesion molecule L-selectin /content/t132186t45251l1v/xxlarge946.gif" alt=" beta" align=" MIDDLE" BORDER=" 0" >2-integrin |
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